Anti-NFM (Neurofilament Medium Chain) Antibody

Name: Anti-NFM (Neurofilament Medium Chain) Antibody
Brand: Aves Labs
SKU: NFM
Price: 275.0 USD
Availability: InStock

from Aves Labs Datasheet

4 Citations

NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. After several boosts, hens were co-boosted with recombinant NFM protein expressed in bacteria. These antibodies have been validated with human, mouse and rat tissues.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

IgY Fraction

Gene Name

NEFM NEF3 NFM

Molecular Weight

150 kDa

Go to Full Product Details

Immunocytochemical staining of NF-M immunoreactivity (green) and Glial Fibrillary Acidic Protein (GFAP, red) in a culture of mouse cortical neurons. The chicken anti-NFM immunostaining was performed using a 1:2000 dilution, and the GFAP immunostaining was performed using a 1:5000 dilution. Photomicrographs from Dr. Gerry Shaw, EnCor Biotechnology, Inc.

Volume: 200 µL

Variant

Price:

$275

Quantity:

Bulk Order Anti-NFM (Neurofilament Medium Chain) Antibody

Product Details

Target

Neurofilament Medium Chain (NFM)

Target Description

Human Neurofilament Medium Chain (NFM) is a 102,448 dalton protein (916 amino acids) expressed in CNS and PNS neurons. This cytoskeletal protein is normally phosphorylated in vivo and its extent of phosphorylation correlates with different states of axonal growth and stabilization. These observations have led to the hypothesis that the NFM protein cross-links adjacent neurofilament strands and contributes to the structural integrity of the axon.

Format

IgY Fraction

Concentration

2 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

NEFM NEF3 NFM

Molecular Weight

150 kDa

Antigen

Recombinant construct containing the C-terminus of the human sequence expressed in Escherichia coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313554

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Neurofilament medium polypeptide (NF-M) (160 kDa neurofilament protein) (Neurofilament 3) (Neurofilament triplet M protein)

UniProt Details

UniProt (Immunogen Species): P07197

Figlia G, Norrmén C, Pereira JA, Gerber D, Suter U. (2017), 'Dual function of the PI3K-Akt-mTORC1 axis in myelination of the peripheral nervous system.' eLife. 10.7554/eLife.29241.
Arevalo-Martin A, Grassner L, Garcia-Ovejero D, Paniagua-Torija B, Barroso-Garcia G, Arandilla AG, Mach O, Turrero A, Vargas E, Alcobendas M, Rosell C, Alcaraz MA, Ceruelo S, Casado R, Talavera F, Palazón R, Sanchez-Blanco N, Maier D, Esclarin A, Molina-Holgado E. (2018), 'Elevated Autoantibodies in Subacute Human Spinal Cord Injury Are Naturally Occurring Antibodies.' Frontiers in Immunology. 10.3389/fimmu.2018.02365.
Lee SJ, Oses-Prieto JA, Kawaguchi R, Sahoo PK, Kar AN, Rozenbaum M, Oliver D, Chand S, Ji H, Shtutman M, Miller-Randolph S, Taylor RJ, Fainzilber M, Coppola G, Burlingame AL, Twiss JL. (2018), 'hnRNPs interacting with mRNA localization motifs define axonal RNA regulons .' Molecular & Cellular Proteomics. 10.1074/mcp.RA118.000603.
Hines TJ, Gao X, Sahu S, Lange MM, Turner JR, Twiss JL, Smith DS. (2018), 'An Essential Postdevelopmental Role for Lis1 in Mice.' eNeuro. 10.1523/ENEURO.0350-17.2018.

Recover password

Create my account

Anti-NFM (Neurofilament Medium Chain) Antibody