NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. After several boosts, hens were co-boosted with recombinant NFM protein expressed in bacteria. These antibodies have been validated with human, mouse and rat tissues.
Primary Antibody
Human, Mouse, Rat
ICC, IHC, WB
Chicken
IgY Fraction
NEFM NEF3 NFM
150 kDa

Immunocytochemical staining of NF-M immunoreactivity (green) and Glial Fibrillary Acidic Protein (GFAP, red) in a culture of mouse cortical neurons. The chicken anti-NFM immunostaining was performed using a 1:2000 dilution, and the GFAP immunostaining was performed using a 1:5000 dilution. Photomicrographs from Dr. Gerry Shaw, EnCor Biotechnology, Inc.
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