Anti-Neurofilament NF-H Antibody (NAP4)

Name: Anti-Neurofilament NF-H Antibody
Brand: PhosphoSolutions
SKU: 1450-NFH
Price: 375.0 USD
Availability: InStock

Our Anti-Neurofilament NF-H primary antibody from PhosphoSolutions is mouse monoclonal. It detects all known mammal and chicken Neurofilament NF-H and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunostaining of human cerebellar cortex showing labeling of NF-H (cat. 1450-NFH, DAB, 1:500) in basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image and the molecular layer at the top. Section was counterstained with Hematoxylin.

Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.

Species Reactivity

Chicken

Applications

ICC, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1450-NFH

Volume: 100 µL

Variant

Price:

$375.00

Quantity:

Product Details

Target

Neurofilament NF-H

Target Description

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3).

Format

Protein G Purified

Clonality

Monoclonal

Clone

NAP4

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

NEFH

Molecular Weight

200 kDa

Antigen

Purified bovine NF-H

Species Reactivity

Chicken

Antibody Registry ID

AB_2492159

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Protein G purified tissue culture supernatant.

Buffer

PBS + 10 mM NaN3.

Dilution Ranges

WB: 1:5000
WB Brain: 1:5000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~200 kDa Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

200 kDa neurofilament protein antibody, CMT2CC antibody, Nefh antibody, Neurofilament heavy polypeptide 200kDa antibody, Neurofilament heavy polypeptide antibody, Neurofilament triplet H protein antibody, NF H antibody, NF-H antibody, NFH antibody, NFH_HUMAN antibody

UniProt Details

UniProt (Human): P12036
UniProt (Immunogen Species): P12036

NCBI Gene ID

4744

Bulk Order Anti-Neurofilament NF-H Antibody

Product Specific Protocols

Western Blot Protocol: Download

General References

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62. PMID: 1724473
Mendonca DM, Chimelli L, Martinez AM. (2005) Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis. Braz J Med Biol Res. 38(6):925-933. PMID: 15933787
Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60. PMID: 11852185

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Anti-Neurofilament NF-H Antibody (NAP4)

SKU: 1450-NFH

Product Details

Bulk Order Anti-Neurofilament NF-H Antibody

Product Specific Protocols

General References

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