Our Anti-NCC (Thiazide sensitve NaCl cotransporter) (Thr53) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NCC (Thiazide sensitve NaCl cotransporter) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Primary Antibody
Mouse
Guinea Pig, Hamster
IHC, WB
Rabbit
Antigen Affinity Purified from Pooled Serum
SLC12A3
160 kDa



Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Product Specific References for Applications and Species
Immunohistochemistry: Human | ||
PMID | Dilution | Publication |
21963515 | not listed | Hoorn, E.J., et al. 2011. The calcineurin inhibitor tacrolimus activates the renal sodium chloride cotransporter to cause hypertension. Nature medicine, 17(10), p.1304. |
Immunohistochemistry: Mouse | ||
PMID | Dilution | Publication |
30517856 | 1:100 | Saritas, T., et al. 2018. Optical Clearing in the Kidney Reveals Potassium-Mediated Tubule Remodeling. Cell reports, 25(10), pp.2668-2675. |
29412704 | 1:10,000 | Terker, A.S., et al. 2018. With no lysine kinase 4 modulates sodium potassium 2 chloride cotransporter activity in vivo. American Journal of Physiology-Renal Physiology, 315(4), pp.F781-F790. |
29263298 | 1:10,000 | Ferdaus, M.Z., et al. 2017. Mutant Cullin 3 causes familial hyperkalemic hypertension via dominant effects. JCI insight, 2(24). |
24799612 | not listed | Terker, A.S., et al. 2014. Sympathetic stimulation of thiazide-sensitive sodium chloride cotransport in the generation of salt-sensitive hypertension. Hypertension, 64(1), pp.178-184. |
22651238 | not listed | Komers, R., et al. 2012. Enhanced phosphorylation of Na+–Cl− co-transporter in experimental metabolic syndrome: role of insulin. Clinical science, 123(11), pp.635-647. |
21963515 | not listed | Hoorn, E.J., et al. 2011. The calcineurin inhibitor tacrolimus activates the renal sodium chloride cotransporter to cause hypertension. Nature medicine, 17(10), p.1304. |
21907141 | 1:500 | McCormick, J.A., et al. 2011. A SPAK isoform switch modulates renal salt transport and blood pressure. Cell metabolism, 14(3), pp.352-364. |
21896937 | not listed | McCormick, J.A., et al. 2011. Overexpression of the sodium chloride cotransporter is not sufficient to cause familial hyperkalemic hypertension. Hypertension, 58(5), 888-894. |
Immunohistochemistry: Rat | ||
PMID | Dilution | Publication |
22651238 | not listed | Komers, R., et al. 2012. Enhanced phosphorylation of Na+–Cl− co-transporter in experimental metabolic syndrome: role of insulin. Clinical science, 123(11), pp.635-647. |
21896937 | not listed | McCormick, J.A., et al. 2011. Overexpression of the sodium chloride cotransporter is not sufficient to cause familial hyperkalemic hypertension. Hypertension, 58(5), 888-894. |
Western Blot: Human | ||
PMID | Dilution | Publication |
29547703 | 1:2000 | Tutakhel, O.A., et al. 2018. Dominant functional role of the novel phosphorylation site S811 in the human renal NaCl cotransporter. The FASEB Journal, pp.fj-201701047R. |
21963515 | not listed | Hoorn, E.J., et al. 2011. The calcineurin inhibitor tacrolimus activates the renal sodium chloride cotransporter to cause hypertension. Nature medicine, 17(10), p.1304. |
Western Blot: Mouse | ||
PMID | Dilution | Publication |
37016934 | 1:2000 | Gao, Z.X., et al. 2023. Kir4.1 Deletion Prevents Salt-Sensitive Hypertension in Early Streptozotocin-Induced Diabetic Mice via Na + -Cl - Cotransporter in the Distal Convoluted Tubule. Journal of Hypertension, 958-970. |
36821372 | 1:200 | Zhang, D.D., et al. 2023. Calcineurin-inhibitors Stimulate Kir4.1/Kir5.1 of Distal-Convoluted-Tubule to Increase Na-Cl Cotransporter (NCC). JCI Insight, e165987. |
35274831 | 1:1000 | Hu, C., et al. 2022. Profiling renal sodium transporters in mice with nephron Ift88 disruption: Association with sex, cysts, and blood pressure. Physiological Reports, 10(5), e15206. |
34029145 | 1:3000 | Xiao, Y., et al. 2021. Deletion of renal Nedd4-2 abolishes the effect of high K+ intake (HK) on Kir4. 1/Kir5. 1 and NCC activity in the distal convoluted tubule. American Journal of Physiology-Renal Physiology, 321(1), F1-F11. |
33900854 | 1:3000 | Duan, X.P., et al. 2021. Deletion of Kir5. 1 abolishes the effect of high-Na+-intake on Kir4. 1 and Na-Cl-cotransporter. American Journal of Physiology-Renal Physiology, 320(6), F1045-F1058. |
33818128 | 1:3000 | Zhang, D.D., et al. 2021. Deletion of renal Nedd4-2 abolishes the effect of high sodium intake (HS) on Kir4. 1, ENaC and NCC, and causes hypokalemia during HS. American Journal of Physiology-Renal Physiology. |
32924546 | 1:2000 | Tahaei, E., et al. 2020. Distal convoluted tubule sexual dimorphism revealed by advanced 3D imaging. American Journal of Physiology-Renal Physiology, 319(5), pp.F754-F764. |
32715760 | 1:1000 | Wang, L.J., et al. 2020. PGF2α stimulates 10-pS Cl channel and thiazide-sensitive Na-Cl cotransporter (NCC) in distal convoluted tubule. American Journal of Physiology-Renal Physiology. |
32633545 | not listed | Gilani, A., et al. 2020. Proximal tubule-targeted overexpression of the Cyp4a12-20-HETE synthase promotes salt-sensitive hypertension in male mice. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 319(1), pp.R87-R95. |
32295826 | not listed | Wu, P., et al. 2020. Renal Tubule Nedd4-2 Deficiency Stimulates Kir4. 1/Kir5. 1 and Thiazide-Sensitive NaCl Cotransporter in Distal Convoluted Tubule. Journal of the American Society of Nephrology, Apr 15; ASN.2019090923. |
31941842 | 1:1000 | Jobbagy, S., et al. 2020. Nrf2 activation protects against lithium-induced nephrogenic diabetes insipidus. JCI Insight, 5(1). |
31239388 | not listed | Wu, P., et al. 2019. . Deletion of Kir5. 1 Impairs Renal Ability to Excrete Potassium during Increased Dietary Potassium Intake. Journal of the American Society of Nephrology, Aug;30(8):1425-1438. |
30728179 | not listed | Khamaysi, A., et al. 2019. Systemic Succinate Homeostasis and Local Succinate Signaling Affect Blood Pressure and Modify Risks for Calcium Oxalate Lithogenesis. Journal of the American Society of Nephrology, pp.ASN-2018030277. |
30571558 | 1:1000 | Duan, X.P., et al. 2019. Norepinephrine-Induced Stimulation of Kir4. 1/Kir5. 1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule. Hypertension,73:112-120. |
30355950 | not listed | Xu, J., et al. 2018. Slc4a8 in the Kidney: Expression, Subcellular Localization and Role in Salt Reabsorption. Cellular Physiology and Biochemistry, 50(4), pp.1361-1375. |
30301860 | 1:2000 | Cornelius, R.J., et al. 2018. Renal COP9 signalosome deficiency alters CUL3-KLHL3-WNK signaling pathway. Journal of the American Society of Nephrology, 29(11), pp.2627-2640. |
30252533 | 1:1000 | Cherezova, A., et al. 2018. Urinary concentrating defect in mice lacking Epac1 or Epac2. The FASEB Journal, pp.fj-201800435R. |
29412704 | 1:2000 | Terker, A.S., et al. 2018. With no lysine kinase 4 modulates sodium potassium 2 chloride cotransporter activity in vivo. American Journal of Physiology-Renal Physiology, 315(4), pp.F781-F790. |
29310825 | 1:6000 | Wang, M.X., et al. 2018. Potassium intake modulates the thiazide-sensitive sodium-chloride cotransporter (NCC) activity via the Kir4. 1 potassium channel. Kidney international, 93(4), pp.893-902. |
29263298 | 1:2000 | Ferdaus, M.Z., et al. 2017. Mutant Cullin 3 causes familial hyperkalemic hypertension via dominant effects. JCI insight, 2(24). |
28052988 | 1:1000 | Cuevas, C.A., et al. 2017. Potassium sensing by renal distal tubules requires Kir4. 1. Journal of the American Society of Nephrology, 28(6), pp.1814-1825. |
27068441 | 1:2000 | Ferdaus, M.Z., et al. 2016. SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule. The Journal of physiology, 594(17), pp.4945-4966. |
26712527 | not listed | Terker, A.S., et al. 2016. Direct and Indirect Mineralocorticoid Effects Determine Distal Salt Transport. J Am Soc Nephrol. (8):2436-45 |
26432904 | 1:6000 | Lazelle, R.A., et al. 2016. Renal deletion of 12 kDa FK506-binding protein attenuates tacrolimus-induced hypertension. Journal of the American Society of Nephrology, 27(5), pp.1456-1464. |
26422504 | not listed | Terker, A.S., et al. 2015. Unique chloride-sensing properties of WNK4 permit the distal nephron to modulate potassium homeostasis. Kidney international, 89(1), pp.127-134. |
25565204 | not listed | Terker, A.S., et al. 2015. Potassium modulates electrolyte balance and blood pressure through effects on distal cell voltage and chloride. Cell Metab. (1):39-50. |
25250572 | not listed | McCormick, J.A., et al. 2014. Hyperkalemic hypertension–associated cullin 3 promotes WNK signaling by degrading KLHL3. The Journal of clinical investigation, 124(11), pp.4723-4736. |
25113964 | not listed | Chávez -Canales, M., et al. 2014. WNK-SPAK-NCC cascade revisited: WNK1 stimulates the activity of the Na-Cl cotransporter via SPAK, an effect antagonized by WNK4. Hypertension, 64(5), pp.1047-1053. |
24799612 | not listed | Terker, A.S., et al. 2014. Sympathetic stimulation of thiazide-sensitive sodium chloride cotransport in the generation of salt-sensitive hypertension. Hypertension, 64(1), pp.178-184. |
24231659 | 1:5000 | Picard, N., et al. 2014. . Protein phosphatase 1 inhibitor-1 deficiency reduces phosphorylation of renal NaCl cotransporter and causes arterial hypotension. Journal of the American Society of Nephrology, 25(3), pp.511-522. |
22651238 | not listed | Komers, R., et al. 2012. Enhanced phosphorylation of Na+–Cl− co-transporter in experimental metabolic syndrome: role of insulin. Clinical science, 123(11), pp.635-647. |
21963515 | not listed | Hoorn, E.J., et al. 2011. The calcineurin inhibitor tacrolimus activates the renal sodium chloride cotransporter to cause hypertension. Nature medicine, 17(10), p.1304. |
21907141 | not listed | McCormick, J.A., et al. 2011. A SPAK isoform switch modulates renal salt transport and blood pressure. Cell metabolism, 14(3), pp.352-364. |
21896937 | not listed | McCormick, J.A., et al. 2011. Overexpression of the sodium chloride cotransporter is not sufficient to cause familial hyperkalemic hypertension. Hypertension, 58(5), 888-894. |
Western Blot: Rat | ||
PMID | Dilution | Publication |
37318990 | 1:1000 | Zietara, A, et al. 2023. Kir7.1 Knockdown and Inhibition Alter Renal Electrolyte Handling But Not the Development of Hypertension in Dahl Salt-sensitive Rats. American Journal of Physiology. Renal Physiology, F177-F187. |
37082243 | not listed | Cai, L., et al. 2023. Dietary Sodium Enhances the Expression of SLC4 Family Transporters, IRBIT, L-IRBIT, and PP1 in Rat Kidney: Insights Into the Molecular Mechanism for Renal Sodium Handling. Frontiers in Physiology, 1154694. |
35466690 | 1:1000 | Kravtsova, O., et al. 2022. SGLT2 inhibition effect on salt-induced hypertension, RAAS, and Na+ transport in Dahl SS rats. American Journal of Physiology, 322(6), ppF692-F707. |
34904226 | not listed | Isaeva, E., et al. 2021. Crosstalk between epithelial sodium channels (ENaC) and basolateral potassium channels (Kir4.1/Kir5.1) in the cortical collecting duct. British Journal of Pharmacology. |
32830328 | 1:500 | Fu, Y., et al. 2020. Geniposide in Gardenia jasminoides var. radicans Makino modulates blood pressure via inhibiting WNK pathway mediated by the estrogen receptors. Journal of Pharmacy and Pharmacology. |
31608673 | 1:1000 | Frame, A.A., et al. 2019. Sympathetic regulation of NCC in norepinephrine-evoked salt-sensitive hypertension in Sprague-Dawley rats. American Journal of Physiology-Renal Physiology, 317(6), pp.F1623-F1636. |
28931751 | not listed | Palygin, O., et al. 2017. Essential role of K ir 5.1 channels in renal salt handling and blood pressure control. JCI Insight, 2(18). |
22651238 | not listed | Komers, R., et al. 2012. Enhanced phosphorylation of Na+–Cl− co-transporter in experimental metabolic syndrome: role of insulin. Clinical science, 123(11), pp.635-647. |
21896937 | not listed | McCormick, J.A., et al. 2011. Overexpression of the sodium chloride cotransporter is not sufficient to cause familial hyperkalemic hypertension. Hypertension, 58(5), 888-894. |
Product Specific Protocols
- Western Blot Protocol: Download