Our Myosin Light Chain (Ser-1) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects bovine, human, mouse, and rat Myosin Light Chain (Ser-1) and is antigen affinity purified. It is great for use in WB, ICC.
Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8) .
Both smooth muscle and nonmuscle myosin II activity is regulated by the phosphorylation state of the myosin regulatory light chain (MLC, MRLC, MLC20, Myl9). Phosphorylation of MLC at Thr-18 and Ser-19 activates myosin II motor activity and increases myosin filament stability. This activation has important roles in various cell motile processes. By contrast, other phosphorylation sites on MLC may inhibit myosin II activity. PKC phosphorylates Ser-1/Ser-2 and Thr-9 in MLC, and this phosphorylation decreases activated myosin II interaction with actin, as well as inhibits MLC interaction with the activation site kinase, myosin light-chain kinase. The Ser-1/Ser-2 region may be the major inhibitory site since Ser-1 is phosphorylated during PDGF-induced stress fiber disassembly and expression of unphosphorylatable MLC20 at the Ser-1/Ser-2 site suppresses this disassembly. Thus, inhibition of myosin II activity through phosphorylation of Ser-1/Ser-2 may have important roles in growth factor-induced reorganization of actomyosin filaments.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
MYL12A
20
Phospho-MLC (Ser-1) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1 in bovine myosin regulatory light chain 9 (Myl9). This peptide sequence is highly conserved in human, rat, and mouse Myl9, and in other smooth muscle and non-muscle MLCs.
Bovine, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
Unconjugated
The antibody detects a 20 kDa* band corresponding to the molecular weight of myosin light chain in human A431 treated with EGF or calyculin A. In addition, the antibody detects the 20 kDa myosin light chain in mouse C2C12, rat PC12, and rabbit fibroblasts treated with calyculin A.
Phosphorylated
Ser-1
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Aguilar-Cuenca, R, et al. 2020. Tyrosine Phosphorylation of the Myosin Regulatory Light Chain Controls Non-muscle Myosin II Assembly and Function in Migrating Cells. Current biology : CB, 2446-2458.
Yadav, P, et al. 2023. EZH2 and matrix co-regulate phenotype and KCNB2 expression in bladder smooth muscle cells. American Journal of Clinical and Experimental Urology, 293-303.
