Anti-MMP9 Antibody (L51/82)

Name: Anti-MMP9 Antibody
Brand: NeuroMab
SKU: 75-100
Price: 329.0 USD
Availability: InStock

Our Anti-MMP9 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L51/82. It detects human, mouse, and rat MMP9, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Immunoblot versus lysates of whole rat brain (WBH) and of COS-1 cells transfected with human and rat MMP9 (hMMP9 and rMMP9, respectively) and Kv2.1 plasmids and probed with L51/82 (left), K89/34 (middle) and loading control (right) TC supe.

Adult rat hippocampus immunohistochemistry.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-100

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

MMP9

Scientific Background

Matrix Metallopeptidase 9 is encoded by the gene MMP9. MMP9 is a member of the peptidase M10A family. MMP9 may play a role in local proteolysis of the extracellular matrix, leukocyte migration, and bone osteoclastic resorption. MMP9 cleaves type IV and V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. MMP9 is expressed in neutrophils. Diseases associated with MMP9 include Metaphyseal Anadysplasia 2 and Metaphyseal Anadysplasia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L51/82

Isotype

IgG2a

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Mmp9

Molecular Weight

92 and 82 kDa (Pro and Active forms)

Antigen

Fusion protein amino acids 1-708 (full-length) of rat MMP9 (accession number P50282) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2297774

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.27

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Matrix metalloproteinase-9 (MMP-9) (EC 3.4.24.35) (92 kDa gelatinase) (92 kDa type IV collagenase) (Gelatinase B) (GELB)

UniProt ID

P50282

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Rat
Western Blot: Human | Mouse
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
24627221	1:200	Duellman, T., et al. 2014. Single nucleotide polymorphism-specific regulation of matrix metalloproteinase-9 by multiple miRNAs targeting the coding exon.. Nucleic Acids Research, 5518-5531.

Immunohistochemistry: Rat
PMID	Dilution	Publication
24114974	1:500	Aujla, P.K., et al. 2014. Early postnatal expression and localization of matrix metalloproteinases-2 and -9 during establishment of rat hippocampal synaptic circuitry.. Journal of Comparative Neurology, 1249-1263.

Western Blot: Human
PMID	Dilution	Publication
28860188	not listed	Scannevin, R.H., et al. 2017. Discovery of a highly selective chemical inhibitor of matrix metalloproteinase-9 (MMP-9) that allosterically inhibits zymogen activation.. The Journal of Biological Chemistry, 17963-17974.
24627221	1:1000	Duellman, T., et al. 2014. Single nucleotide polymorphism-specific regulation of matrix metalloproteinase-9 by multiple miRNAs targeting the coding exon.. Nucleic Acids Research, 5518-5531.
22942228	1:1000	Duellman, T., et al. 2012. Matrix metalloproteinase-9 genotype as a potential genetic marker for abdominal aortic aneurysm.. Circulation. Cardiovascular Genetics, 529-537.
22749475	1:500	Delfín, D.A., et al. 2012. Cardiomyopathy in the dystrophin/utrophin-deficient mouse model of severe muscular dystrophy is characterized by dysregulation of matrix metalloproteinases.. Neuromuscul Disord., 1006-1014.

Western Blot: Mouse
PMID	Dilution	Publication
29439712	not listed	Garg, C., et al. 2018. Trovafloxacin attenuates neuroinflammation and improves outcome after traumatic brain injury in mice. Journal of Neuroinflammation, 42.
21703213	not listed	Moore, B.A., et al. 2012. Matrix metalloproteinase-9 inhibition reduces inflammation and improves motility in murine models of postoperative ileus.. Gastroenterology, 1283-1292.

Additional Publications: Unspecified
PMID	Publication
23606560	Cayabyab, F.S., et al. 2013. Involvement of matrix metalloproteinases-2 and -9 in the formation of a lacuna-like cerebral cavity.. Journal of Neuroscience Research, 920-933.
20008515	DeNiro, M., et al. 2010. Pleiotropic effects of YC-1 selectively inhibit pathological retinal neovascularization and promote physiological revascularization in a mouse model of oxygen-induced retinopathy.. Molecular Pharmacology, 348-367.

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Anti-MMP9 Antibody (L51/82)

SKU: 75-100

Product Details

Product Specific References for Applications and Species

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