Anti-mCherry Antibody
Our Anti-mCherry chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects mCherry, and is affinity purified. It is great for use in ICC, WB.
SKU: MCHERRY-0100
Product Details
mCherry
mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.
Affinity purified
0.2 mg/mL
Polyclonal
IgY
ICC, WB
Chicken
MCHERRY
27 KDa for native monomer
Purified recombinant mCherry (Uniprot X5DSL3) expressed in E.Coli
AB_2910557
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
WB: 1:500-1:1000
ICC: 1:100-1:500
ICC: 1:100-1:500
Each new lot of this antibody is tested by Western blot on an mCherry overexpression cell lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
Ice packs
UniProt (Immunogen Species): X5DSL3
Product Specific References for Applications and Species
Immunocytochemistry: Human | ||
PMID | Dilution | Publication |
35941369 | 1:1000 | Sahu, S.K., et al. 2022. A complex epigenome-splicing crosstalk governs epithelial-to-mesenchymal transition in metastasis and brain development. Nature Cell Biology, 1265-1277. |
Western Blot: Rat | ||
PMID | Dilution | Publication |
38081810 | 1:1000 | Zhang, W., et al. 2023. Immunoproximity biotinylation reveals the axon initial segment proteome. Nature Communications, 8201. |