Anti-mCherry Antibody

Name: Anti-mCherry Antibody
Brand: Aves Labs
SKU: MCHERRY-0100
Price: 440.0 USD
Availability: InStock

from Aves Labs Datasheet Trial Size

1 Citation

Our anti-mCherry chicken primary antibody from AvesLabs is manufactured in-house. It detects mCherry, dsRED, RFP (all tested) and tdTOMATO (by similarity) and is purified. It is provided in liquid form.

Product Type

Primary Antibody

Applications

ICC, WB

Host Species

Chicken

Format

Affinity purified

Gene Name

MCHERRY

Molecular Weight

27 KDa for native monomer

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Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.

Western blotting of recombinant fluorescent proteins with AvesLabs Chicken anti- mCherry antibody. The indicated (nanogram) amounts of each purified recombinant fluorescent protein was loaded on a gel and analyzed by Western blotting. AvesLabs Chicken anti-mCherry antibody recognizes ng amounts of mCherry protein and the related dsRED protein but does not show any reactivity with GFP.

Western blot of COS7 cells transfected with mCherry-myc-FLAG (lane 1), GFP (lane 2), or mock transfected cells (lane 3) using chicken α-mCherry antibody showing the expected staining for mCherry in lane 1 and no staining in GFP or untransfected cells. The ~37kDa major band in lane 1 represents full-length mCherry-myc-FLAG while the shorter ~24kDa band represents an mCherry degradation product.

Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.

Volume: 500 µL

Variant

Price:

$440

Quantity:

Bulk Order Anti-mCherry Antibody

Product Details

Target

mCherry

Target Description

mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.

Format

Affinity purified

Concentration

0.2 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, WB

Host Species

Chicken

Gene Name

MCHERRY

Molecular Weight

27 KDa for native monomer

Antigen

Purified recombinant mCherry (Uniprot X5DSL3) expressed in E.Coli

Antibody Registry ID

AB_2910557

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:500-1:1000
ICC: 1:100-1:500

Quality Control

Each new lot of this antibody is tested by Western blot on an mCherry overexpression cell lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

Ice packs

UniProt Details

UniProt (Immunogen Species): X5DSL3

Product Specific References for Applications and Species

Immunocytochemistry: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
35941369	1:1000	Sahu, S.K., et al. 2022. A complex epigenome-splicing crosstalk governs epithelial-to-mesenchymal transition in metastasis and brain development. Nature Cell Biology, 1265-1277.

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Anti-mCherry Antibody

Our anti-mCherry chicken primary antibody from AvesLabs is manufactured in-house. It detects mCherry, dsRED, RFP (all tested) and tdTOMATO (by similarity) and is purified. It is provided in liquid form.