Anti-Lamin A/C Antibody

Name: Anti-Lamin A/C Antibody
Brand: Biosensis
SKU: R-1697-100
Price: 327.0 USD
Availability: InStock

Our Anti-Lamin A/C rabbit polyclonal primary antibody detects human, other mammals and (predicted) Lamin A/C, and is affinity-purified. It is validated for use in ICC, WB.

Crude HeLa cell extract stained with Rabbit anti-Lamin A/C. Two bands at 74 kDa and 65 kDa, corresponding to Lamin A and C.

HeLa cells stained with R-1697-100 (red), and counterstained with our monoclonal antibody to HSP27 (M-1692-100). The Lamin A/C antibody reveals strong nuclear lamina staining, while the HSP27 antibody reveals strong cytoplasmic staining. The blue stain reveals DNA in the nuclei of these cells.

Crude HeLa cell extract stained with Rabbit anti-Lamin A/C. Two bands at 74 kDa and 65 kDa, corresponding to Lamin A and C.

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Species Reactivity

Human

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1697-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Lamin A/C

Scientific Background

The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm (1). The lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene (2), while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74 kDa while Lamin C is 65 kDa. The Lamin A protein includes 98 amino acids missing from Lamin C, while Lamin C has a C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other, as is the case with this monoclonal. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging (3).

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Molecular Weight

74 kDa and 65 kDa

Immunogen

Full length recombinant human Lamin C

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized with sodium azide.

Dilution Ranges

WB: 1:1000-1:5000
ICC: 1:1000-1:5000

Conjugate

Unconjugated

Specificity

Lamin A and Lamin C. The antibody reacts with a 74 kDa and 65 kDa band by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry on HeLa cell cultures.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

UniProt ID

P02545

Shipping

25°C (ambient)

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Anti-Lamin A/C Antibody

SKU: R-1697-100

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