Anti-Kv2.2 K+ Channel Antibody (K37/89)

Name: Anti-Kv2.2 K+ Channel Antibody
Brand: NeuroMab
SKU: 75-015
Price: 319.0 USD
Availability: InStock

11 Citations

Our Anti-Kv2.2 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K37/89. It is KO validated, detects human, mouse, rabbit, and rat Kv2.2 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Adult rat basal forebrain immunofluorescence with antigen retrieval via sodium citrate pretreatment with K37/89 (red) and Kv2.1 rabbit (green). Image courtesy of Kaori Misonou and Hiroaki Misonou (University of Maryland, now at Doshisha University, Japan).

Immunoblot against adult rat brain membranes (RBM) and extracts of COS cells transiently transfected with Myc-tagged Kv2.2short, GFP-tagged Kv2.2long or untagged Kv2.1 plasmid probed with K37/89 (left), K89/34 (middle) or N52A/42 (right) TC supe.

Immunoblot against adult rat brain membranes (RBM) and membranes from Kv2.2 wild-type (WT) and knockout (KO) mice probed with K37/89 (left) or K89/34 TC supe. Mouse brains courtesy of Amy Huntley and Jeanne Nerbonne (Washington University).

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with K37/89 TC supe.$

Adult rat basal forebrain immunofluorescence with antigen retrieval via sodium citrate pretreatment with K37/89 (red) and Kv2.1 rabbit (green). Image courtesy of Kaori Misonou and Hiroaki Misonou (University of Maryland, now at Doshisha University, Japan).

Species Reactivity

Human, Mouse, Rabbit, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-015

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Kv2.2 K+ channel

Target Description

Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K37/89

Isotype

IgG2a

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

KCNB2

Molecular Weight

125 kDa

Antigen

Fusion protein amino acids 1-61 of rat Kv2.2 (accession number Q63099) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rabbit, Rat

Antibody Registry ID

AB_2131665

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Potassium voltage-gated channel subfamily B member 2 (Voltage-gated potassium channel subunit Kv2.2)

UniProt Details

UniProt (Human): Q92953
UniProt (Immunogen Species): Q92953

Product Specific References for Applications and Species

Immunocytochemistry: Rabbit
Immunohistochemistry: Rat
Immunoprecipitation: Rat
Western Blot: Mouse | Rat

Immunocytochemistry: Rabbit
PMID	Dilution	Publication
21813710	not listed	Kyle, B., et al. 2011. Contribution of Kv2. 1 channels to the delayed rectifier current in freshly dispersed smooth muscle cells from rabbit urethra. . American Journal of Physiology-Cell Physiology, C1186-C1200.

Immunohistochemistry: Rat
PMID	Dilution	Publication
24723263	1:100	Dufour, M.A., et al. 2014. Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development.. Journal of Neuroscience Research, 981-99.
23197740	1:1000	Tsantoulas, C., et al. 2012. Sensory neuron downregulation of the Kv9.1 potassium channel subunit mediates neuropathic pain following nerve injury.. Journal of Neuroscience, 17502-13.
20853508	not listed	Hermanstyne, T.O., et al. 2010. Immunolocalization of the voltage-gated potassium channel Kv2.2 in GABAergic neurons in the basal forebrain of rats and mice.. Journal of Comparative Neurology, 4298-310.
20202934	not listed	Kihira, Y., et al. 2010. Formation of heteromeric Kv2 channels in mammalian brain neurons. . Journal of Biological Chemistry, 15048-15055.

Immunoprecipitation: Rat
PMID	Dilution	Publication
34137443	1 ug	Maverick, E.E., et al. 2021. Kv2 channel–AMIGO β-subunit assembly modulates both channel function and cell adhesion molecule surface trafficking. . Journal of cell science, jcs256339.

Western Blot: Mouse
PMID	Dilution	Publication
24494598	not listed	Speca, D.J., et al. 2014. Deletion of the Kv2.1 delayed rectifier potassium channel leads to neuronal and behavioral hyperexcitability.. Genes, Brain and Behavior, 394-408.
20853508	not listed	Hermanstyne, T.O., et al. 2010. Immunolocalization of the voltage-gated potassium channel Kv2.2 in GABAergic neurons in the basal forebrain of rats and mice.. Journal of Comparative Neurology, 4298-310.

Western Blot: Rat
PMID	Dilution	Publication
33803465	1:1000	Zhang, Y., et al. 2021. Regulation of K+ Conductance by a Hydrogen Bond in Kv2. 1, Kv2. 2, and Kv1. 2 Channels.. Membranes, 190.
24597762	1:1000	Wang, C.Y., et al. 2014. GDF15 regulates Kv2. 1-mediated outward K+ current through the Akt/mTOR signalling pathway in rat cerebellar granule cells. . Biochemical Journal, 35-47.
21412780	1:500	Zhuang, J.L., et al. 2011. TGF‐β1 enhances Kv2. 1 potassium channel protein expression and promotes maturation of cerebellar granule neurons. . Journal of cellular physiology, 297-307.

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Anti-Kv2.2 K+ Channel Antibody (K37/89)

SKU: 75-015

Product Details

Product Specific References for Applications and Species

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