Anti-Green Fluorescent Protein (GFP) Antibody

Our Anti-Green Fluorescent Protein (GFP) chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects n/a Green Fluorescent Protein (GFP), and is mixture of IgY fraction and affinity-purified antibodies. Works in ELISA, IHC, ICC, WB.

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).
Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.
Comparison between GFP immunoreactivity in Rexed Lamina 2 neurons of a transgenic mouse using Aves Labs’ anti-GFP (green) and rabbit anti-GFP (red). The transgenic mouse was generated by placing the GFP cDNA after a POMC gene promoter in the transfected plasmid. Mark Zilka, Univ. North Carolina.
Comparison between GFP-immunoreactivity using Aves' anti-GFP antibody (left panel in red) and autofluorescence (right panel in green). In this case, the cortical neuron in this unfixed thick section was first photographed for GFP autofluorescence (left), and then the section was fixed (4% paraformaldehyde) and immunostained for GFP-immunoreactivity (1:1000 dilution) using Texas Red-goat anti-chicken IgY antibodies (Jackson ImmunoResearch) as a secondary. The same cell (left) was then identified.
Immunoreactivity using anti-GFP antibody (left panel in red), Western blot showing specific immunolabeling of the GFP protein.
Immunolabeling of dividing Drosophilia larval neuroblast using Anti-GFP (green, Cat. No. GFP-1010, 1:500) to detect the GFP tagged telomere protein HOAP, and nuclei stained with DAPI (grey). Image was taken with a Zeiss 880 confocal and kindly provided by Brandt Warecki, Sullivan Lab, University of California, Santa Cruz.
Immunostaining of mouse hippocampus identifying injected HSV containing GFP (Cat. GFP, 1:250, green) and DAPI (blue). Image kindly provided by Chad Brunswick, Penn State University.
Immunostaining of adult hippocampal neural stem cells from Nestin GFP trangenic mice labeling GFAP (orange, mouse) and GFP (orange, 1:1000, chicken,Cat. GFP, GFP). The confocal image was obtained from a Leica Sp8 confocal microscope, magnification 40X. Image kindly provided by Soraya Martín Suarez, Achucarro Basque Center for Neuroscience, Spain.
Immunostaining of CX3CR1-Cre mouse hypothalamus showing specific detection of GFP (Cat. GFP, green) and IBA1 (red). Nuclei are labeled with DAPI (blue). Image kindly provided by Karmen Ma, NIDDK – National Institutes of Health.
Immunostaining of CX3CR1-Cre mouse hypothalamus showing specific detection of GFP (Cat. GFP, green) and IBA1 (red). Image kindly provided by Karmen Ma, NIDDK – National Institutes of Health.
Immunostaining of mouse primary astrocyte cultures expressing a GFP tagged GPCR, stained with GFP (Cat. GFP, 1:2500). Image kindly provided by Sandeep Singh, Virginia Commonwealth University.
Representative image defining the following zebrafish microglial morphologies: ramified, cup formation, and soma retraction. White arrowheads show interactions between the microglial process (mpeg:EGFP-CAAX, Cat no GFP, green, 1:1000) and surrounding neuronal cell bodies (NBT:dsRed). Image from publication CC-BY-4.0. PMID: 38490196
Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).
Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).
Click on image to zoom

ELISA, ICC, IHC, WB
Chicken

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Data Sheet

SKU: GFP-1020

Volume: 400 µL
Price:
$527.00
Ships: 1-2 business days

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