Anti-Green Fluorescent Protein Antibody

Name: Anti-Green Fluorescent Protein Antibody
Brand: Aves Labs
SKU: GFP-1020
Price: 485.0 USD
Availability: InStock

from Aves Labs Datasheet

2153 Citations

Product Type

Primary Antibody

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Format

IgY Fraction

Molecular Weight

27 kDa

Go to Full Product Details

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).

Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.

Comparison between GFP immunoreactivity in Rexed Lamina 2 neurons of a transgenic mouse using Aves Labs’ anti-GFP (green) and rabbit anti-GFP (red). The transgenic mouse was generated by placing the GFP cDNA after a POMC gene promoter in the transfected plasmid. Mark Zilka, Univ. North Carolina.

Comparison between GFP-immunoreactivity using Aves' anti-GFP antibody (left panel in red) and autofluorescence (right panel in green). In this case, the cortical neuron in this unfixed thick section was first photographed for GFP autofluorescence (left), and then the section was fixed (4% paraformaldehyde) and immunostained for GFP-immunoreactivity (1:1000 dilution) using Texas Red-goat anti-chicken IgY antibodies (Jackson ImmunoResearch) as a secondary. The same cell (left) was then identified.

Immunoreactivity using anti-GFP antibody (left panel in red), Western blot showing specific immunolabeling of the GFP protein.

Immunolabeling of dividing Drosophilia larval neuroblast using Anti-GFP (green, Cat. No. GFP-1010, 1:500) to detect the GFP tagged telomere protein HOAP, and nuclei stained with DAPI (grey). Image was taken with a Zeiss 880 confocal and kindly provided by Brandt Warecki, Sullivan Lab, University of California, Santa Cruz.

Immunostaining of mouse hippocampus identifying injected HSV containing GFP (Cat GFP, 1:250, green) and DAPI (blue). Image kindly provided by Chad Brunswick, Penn State University.

Immunostaining of adult hippocampal neural stem cells from Nestin GFP trangenic mice labeling GFAP (orange, mouse) and GFP (orange, 1:1000, chicken, cat co GFP). The confocal image was obtained from a Leica Sp8 confocal microscope, magnification 40X. Image kindly provided by Soraya Martín Suarez, Achucarro Basque Center for Neuroscience, Spain.

Immunostaining of CX3CR1-Cre mouse hypothalamus showing specific detection of GFP (cat. GFP, green) and IBA1 (red). Nuclei are labeled with DAPI (blue). Image kindly provided by Karmen Ma, NIDDK – National Institutes of Health.

Immunostaining of CX3CR1-Cre mouse hypothalamus showing specific detection of GFP (cat. GFP, green) and IBA1 (red). Image kindly provided by Karmen Ma, NIDDK – National Institutes of Health.

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).

Volume: 400 µL

Variant

Price:

$485

Quantity:

Bulk Order Anti-Green Fluorescent Protein Antibody

Product Details

Target

Green Fluorescent Protein (GFP)

Target Description

Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.

Format

IgY Fraction

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Molecular Weight

27 kDa

Antigen

Recombinant GFP expressed in Escherichia coli

Antibody Registry ID

AB_2307313

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Physical State

Liquid

Production Notes

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a filter-sterilized mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL).

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product.

Shipping

Ice packs

Product Specific References for Applications and Species

FISH
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Western Blot

FISH
PMID	Dilution	Publication
37696852	not listed	Latallo, MJ, et al. 2023. Single-molecule imaging reveals distinct elongation and frameshifting dynamics between frames of expanded RNA repeats in C9ORF72-ALS/FTD. Nature Communications, 5581.
37500778	1:1000	Wang, WX, et al. 2023. Universal, label-free, single-molecule visualization of DNA origami nanodevices across biological samples using origamiFISH. Nature nanotechnology, 0.

Flow Cytometry
PMID	Dilution	Publication
34732708	1:500	Jarmas, A.E., et al. 2021. Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis. Nature Communications, 6332.

Immunocytochemistry
PMID	Dilution	Publication
37865312	1:500	Benner, O, et al. 2023. Multiple N-linked glycosylation sites critically modulate the synaptic abundance of Neuroligin isoforms. The Journal of Biological Chemistry, 105361.
37858465	1:1000	Hoi, KK, et al. 2023. Primary cilia control oligodendrocyte precursor cell proliferation in white matter injury via Hedgehog-independent CREB signaling. Cell Reports, 113272.
37844219	1:1000	Dingal, PCDP, et al. 2023. Molecular mechanisms controlling the biogenesis of the TGF-β signal Vg1. PNAS USA, e2307203120.
37802038	not listed	Bershteyn, M, et al. 2023. Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy. Cell stem cell, 1331-1350.e11.
37551982	1:1200	Yammine, SZ, et al. 2023. Fate Specification of GFAP-Negative Primitive Neural Stem Cells and Their Progeny at Clonal Resolution. Stem Cells and Development, 606-621.
37535603	not listed	Perlee, S, et al. 2023. SETD1A function in leukemia is mediated through interaction with mitotic regulators BuGZ/BUB3. EMBO Reports, e57108.
37470786	1:1000	Popova, G, et al. 2023. Rubella virus tropism and single-cell responses in human primary tissue and microglia-containing organoids. eLife, .
37442236	1:2000	Dong, R, et al. 2023. The Translation Initiating Factor eIF4E and Arginine Methylation Underlie G3BP1 Function in Dendritic Spine Development of Neurons. The Journal of Biological Chemistry, 105029.
37366790	1:300	Menon, R, et al. 2023. Human Induced Pluripotent Stem Cell-Derived Pericytes as Scalable and Editable Source to Study Direct Lineage Reprogramming Into Induced Neurons. Cellular Reprogramming, .
37329884	1:1000	Livingston, NM, et al. 2023. Bursting Translation on Single mRNAs in Live Cells. Molecular Cell, 2276-2289.
37083045	1:1000	Fan, W., et al. 2023. The Transcriptional Co-Activator Yap1 Promotes Adult Hippocampal Neural Stem Cell Activation. The EMBO Journal, e110384.
36989136	not listed	Sidhaye, J., et al. 2023. Integrated Transcriptome and Proteome Analysis Reveals Posttranscriptional Regulation of Ribosomal Genes in Human Brain Organoids. eLife, .
36965484	1:500-1:1000	Tani, S., et al. 2023. Stem Cell-Based Modeling and Single-Cell Multiomics Reveal Gene-Regulatory Mechanisms Underlying Human Skeletal Development. Cell Reports, 112276.
36880745	not listed	Jansen, K.I., et al. 2023. A Live-Cell Marker to Visualize the Dynamics of Stable Microtubules Throughout the Cell Cycle. The Journal of Cell Biology, .
36800994	1:1000	Kawaue, T., et al. 2023. Inhomogeneous Mechanotransduction Defines the Spatial Pattern of Apoptosis-Induced Compensatory Proliferation. Developmental Cell, 267-277.
36732073	1:1000	Mut-Arbona, P., et al. 2023. Dual Role of the P2X7 Receptor in Dendritic Outgrowth during Physiological and Pathological Brain Development. The Journal of Neuroscience, 1125-1142.
36731471	1:1000	Dave, P., et al. 2023. Single-molecule imaging reveals translation-dependent destabilization of mRNAs. Molecular Cell, .
36717740	1:1000	Bonnet, M., et al. 2023. Pathogenic TRIO variants associated with neurodevelopmental disorders perturb the molecular regulation of TRIO and axon pathfinding in vivo. Molecular Psychiatry, .
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36701233	1:1000	Gallagher, E.R., et al. 2023. The selective autophagy adaptor p62/SQSTM1 forms phase condensates regulated by HSP27 that facilitate the clearance of damaged lysosomes via lysophagy. Cell Reports, 112037.
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35760790	1:400	DeVeale, B., et al. 2022. G1/S restriction point coordinates phasic gene expression and cell differentiation. Nature Communications, 3696.
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35613619	1:1000	Xie, Q., et al. 2022. Transcription factor Acj6 controls dendrite targeting via a combinatorial cell-surface code. Neuron, 2299-2314.
35589708	1:1000	Janota, C.S., et al. 2022. Shielding of actin by the endoplasmic reticulum impacts nuclear positioning. Nature Communications, 2763.
35535491	1:200	Solman, M., et al. 2022. Inflammatory response in hematopoietic stem and progenitor cells triggered by activating SHP2 mutations evokes blood defects. eLife, .
35443165	1:1000	Sênos Demarco, R., et al. 2022. Escargot controls somatic stem cell maintenance through the attenuation of the insulin receptor pathway in Drosophila. Cell Reports, 110679.
35411077	1:1000	Monteiro, C., et al. 2022. Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism. Nature Medicine, 752-765.
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35352947	1:500	Powers, R.M., et al. 2022. Growth cone macropinocytosis of neurotrophin receptor and neuritogenesis are regulated by neuron navigator 1. Molecular Biology of the Cell, ar64.

Immunohistochemistry
PMID	Dilution	Publication
37898094	1:1000	Silva, MSB, et al. 2023. Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female mice. EBioMedicine, 104850.
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37882674	1:400	Chaudhary, P, et al. 2023. Activation of KrasG12D in subset of alveolar Type II cells enhances cellular plasticity in lung adenocarcinoma. Cancer Research Communications, 2400-2411.
37858465	1:1000	Hoi, KK, et al. 2023. Primary cilia control oligodendrocyte precursor cell proliferation in white matter injury via Hedgehog-independent CREB signaling. Cell reports, 113272.
37848288	1ug/ml	Hara, Y, et al. 2023. ADP ribosylation factor 4 (Arf4) regulates radial migration through N-cadherin trafficking during cerebral cortical development. eNeuro, .
37843554	1:500	Ren, L, et al. 2023. Adjudin improves beta cell maturation, hepatic glucose uptake and glucose homeostasis. Diabetologia, .
37838794	1:1000	Shimizu, T, et al. 2023. Oligodendrocyte dynamics dictate cognitive performance outcomes of working memory training in mice. Nature communications, 6499.
37820723	1:500	Tuncdemir, SN, et al. 2023. Adult-born granule cells facilitate remapping of spatial and non-spatial representations in the dentate gyrus. Neuron, .
37819757	1:1000	Kim, HJ, et al. 2023. GABAergic-like dopamine synapses in the brain. Cell Reports, 113239.
37795949	1:500	Mihajlović, AI, et al. 2023. Spindle assembly checkpoint insensitivity allows meiosis-II despite chromosomal defects in aged eggs. EMBO Reports, e57227.
37792535	1:1500	Gui, Y, et al. 2023. Melanocortin-3 receptor expression in AgRP neurons is required for normal activation of the neurons in response to energy deficiency. Cell Reports, 113188.
37742121	1:250	SheikhBahaei, S, et al. 2023. Contributions of carotid bodies, retrotrapezoid nucleus neurons and preBötzinger complex astrocytes to the CO2 -sensitive drive for breathing. The Journal of Physiology, .
37724425	1:500	Ortega-Gascó, A, et al. 2023. Regulation of young-adult neurogenesis and neuronal differentiation by neural cell adhesion molecule 2 (NCAM2). Cerebral Cortex, 10931-10948.
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37735498	1:1000	Hong, J, et al. 2023. Prefrontal cortical regulation of REM sleep. Nature Neuroscience, 1820-1832.
37716349	1:500	Mittelstadt, JK, et al. 2023. Orbitofrontal cortex conveys stimulus and task information to the auditory cortex. Current Biology, .
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37684234	1:1000	Ulloa Severino, FP, et al. 2023. Training-induced circuit-specific excitatory synaptogenesis in mice is required for effort control. Nature Communications, 5522.
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37673072	1:500	Andrews, MG, et al. 2023. LIF signaling regulates outer radial glial to interneuron fate during human cortical development. Cell Stem Cell, .
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37681879	1:500	Veithen, M, et al. 2023. Sox9 Inhibits Cochlear Hair Cell Fate by Upregulating Hey1 and HeyL Antagonists of Atoh1. Cells, .
37664580	1:3000	Bjornson, KJ, et al. 2023. Stress-mediated dysregulation of the Rap1 small GTPase impairs hippocampal structure and function. iScience, 107566.
37648452	1:1000	Allard, S, et al. 2023. Cholinergic reinforcement signaling is impaired by amyloidosis prior to its synaptic loss. The Journal of Neuroscience, .
37625855	1:5000	Dos Santos, WO, et al. 2023. Growth hormone action in somatostatin neurons regulates anxiety and fear memory. The Journal of Neuroscience, .
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37620160	1:1000	Fekete, CD, et al. 2023. Cleavage of VAMP2/3 affects oligodendrocyte lineage development in the developing mouse spinal cord. The Journal of Neuroscience, .
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37595046	1:500	Mi, J, et al. 2023. Decoding pancreatic endocrine cell differentiation and β cell regeneration in zebrafish. Science Advances, eadf5142.
37590333	1:2000	Walker, LJ, et al. 2023. Target-selective vertebrate motor axon regeneration depends on interaction with glial cells at a peripheral nerve plexus. PLoS Biology, e3002223.
37585461	not listed	Vincent, E, et al. 2023. Ret deficiency decreases neural crest progenitor proliferation and restricts fate potential during enteric nervous system development. PNAS: USA, e2211986120.
37567873	1:500	Cigliola, V, et al. 2023. Spinal cord repair is modulated by the neurogenic factor Hb-egf under direction of a regeneration-associated enhancer. Nature Communications, 4857.
37567873	1:500	Cigliola, V, et al. 2023. Spinal cord repair is modulated by the neurogenic factor Hb-egf under direction of a regeneration-associated enhancer. Nature Communications, 4857.
37541195	1:1000	Wolfson, RL, et al. 2023. DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon. Cell, 3368-3385.e18.
37540708	1:1000	Wong, W, et al. 2023. An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold. PLoS Biology, e3002212.
37539750	1:500	Shen, Y, et al. 2023. Uncovering the Heterogeneity of Cardiac Lin -KIT + Cells: A scRNA-seq Study on the Identification of Subpopulations. Stem Cells, .
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37527657	1:2500	Byun, YG, et al. 2023. Stress induces behavioral abnormalities by increasing expression of phagocytic receptor, MERTK, in astrocytes to promote synapse phagocytosis. Immunity, 0.
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37490921	1:1000	Laing, BT, et al. 2023. Anterior Hypothalamic Parvalbumin Neurons are Glutamatergic and Promote Escape Behavior. Current Biology, 3215-3228.
37463889	1:500	Kim, W, et al. 2023. An optimized Nurr1 agonist provides disease-modifying effects in Parkinson's disease models. Nature Communications, 4283.
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37435939	1:5000	Evans, MC, et al. 2023. Leptin, but not Estradiol, Signaling in PACAP Neurons Modulates Puberty Onset. Endocrinology, 0.
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37217726	1:1000	Cury, KM, et al. 2023. Flexible Neural Control of Transition Points Within the Egg-laying Behavioral Sequence in Drosophila. Nature Neuroscience, 1054-1067.
37217517	1:500	Hérent, C, et al. 2023. Upregulation of Breathing Rate During Running Exercise by Central Locomotor Circuits in Mice. Nature Communications, 2939.
37216404	1:1000	Lee, SG, et al. 2023. Taste and Pheromonal Inputs Govern the Regulation of Time Investment for Mating by Sexual Experience in Male Drosophila Melanogaster. PLoS Genetics, e1010753.
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37211631	1:800	Biancardi, V, et al. 2023. Cholinergic Projections to the PreBötzinger Complex. The Journal of Comparative Neurology, 1317-1332.
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37208933	1:1000	Pukos, N, et al. 2023. Chronic Demyelination and Myelin Repair After Spinal Cord Injury in Mice: A Potential Link for Glutamatergic Axon Activity. Glia, 2093-2116.
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37191285	1:1000	Parab, S, et al. 2023. Local Angiogenic Interplay of Vegfc/d and Vegfa Controls Brain Region-specific Emergence of Fenestrated Capillaries. eLife, .
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37117012	1:500	Li, M., et al. 2023. Hypothalamic Supramammillary Nucleus Selectively Excites Hippocampal CA3 Interneurons to Suppress CA3 Pyramidal Neuron Activity. The Journal of Neuroscience, .
37113078	1:500	Ye, F., et al. 2023. Characteristics of Activation of Monocyte-Derived Macrophages Versus Microglia After Mouse Experimental Intracerebral Hemorrhage. Journal of Cerebral Blood Flow and Metabolism, 271678X231173187.
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37100373	1:1000	Singhal, P., et al. 2023. Characteristics of Electrical Synapses, C-terminals and Small-conductance Ca2+ activated Potassium Channel 3 (K+) Channels in the Sexually Dimorphic Cremaster Motor Nucleus in the Spinal Cord of Mouse and Rat. Neuroscience, .
37083045	1:1000	Fan, W., et al. 2023. The Transcriptional Co-Activator Yap1 Promotes Adult Hippocampal Neural Stem Cell Activation. The EMBO Journal, e110384.
37061022	1:1000	Arshad, M.N., et al. 2023. Altered Connectomes of Adult-Born Granule Cells Following Engraftment of GABAergic Progenitors in the Mouse Hippocampus. Progress in Neurobiology, 102450.
37054704	1:1000	Sun, X.L., et al. 2023. Stem Cell Competition Driven by the Axin2-p53 Axis Controls Brain Size During Murine Development. Developmental Cell, 744-759.
37020025	1:1000	Yang, T., et al. 2023. Plastic and Stimulus-Specific Coding of Salient Events in the Central Amygdala. Nature, 510-519.
36991128	1:250	Hu, J., et al. 2023. STING Inhibits the Reactivation of Dormant Metastasis in Lung Adenocarcinoma. Nature, 806-813.
37130522	not listed	Torow, N., et al. 2023. M Cell Maturation and cDC Activation Determine the Onset of Adaptive Immune Priming in the Neonatal Peyer's Patch. Immunity, .
36972315	1:2000	Taniguchi, K., et al. 2023. Sas-Ptp10D Shapes Germ-Line Stem Cell Niche by Facilitating JNK-Mediated Apoptosis. PLoS Genetics, e1010684.
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Western Blot
PMID	Dilution	Publication
37865312	1:500	Benner, O, et al. 2023. Multiple N-linked glycosylation sites critically modulate the synaptic abundance of Neuroligin isoforms. The Journal of Biological Chemistry, 105361.
37844219	1:1000	Dingal, PCDP, et al. 2023. Molecular mechanisms controlling the biogenesis of the TGF-β signal Vg1. PNAS USA, e2307203120.
37596792	1:1000	Mitsou, I, et al. 2023. Two Transient Receptor Potential Channels at Focal Adhesions. The Journal of Histochemistry and Cytochemistry, 221554231194119.
37333770	1:2000	Padgett, LR, et al. 2023. Deoxyhypusine Synthase Mutations Alter the Post-translational Modification of Eukaryotic Initiation Factor 5A Resulting in Impaired Human and Mouse Neural Homeostasis. HGG Advances, 100206.
35163711	not listed	Clarke, B.E., et al. 2022. Enhanced Expression of TRAP1 Protects Mitochondrial Function in Motor Neurons under Conditions of Oxidative Stress. International Journal of Molecular Sciences, 1789.

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Anti-Green Fluorescent Protein Antibody