Anti-Green Fluorescent Protein Antibody

Name: Anti-Green Fluorescent Protein Antibody
Brand: Aves Labs
SKU: GFP-1020
Price: 500.0 USD
Availability: InStock

2297 Citations

Our Anti-Green Fluorescent Protein (GFP) chicken polyclonal primary antibody from Aves Labs is produced in-house and detects Green Fluorescent Protein (GFP) and related variants including eGFP, YFP, CFP and others. It is a mixture of IgY fraction and affinity-purified antibodies and is great for use in ELISA, IHC, ICC, and WB.

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).

Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.

Comparison between GFP immunoreactivity in Rexed Lamina 2 neurons of a transgenic mouse using Aves Labs’ anti-GFP (green) and rabbit anti-GFP (red). The transgenic mouse was generated by placing the GFP cDNA after a POMC gene promoter in the transfected plasmid. Mark Zilka, Univ. North Carolina.

Comparison between GFP-immunoreactivity using Aves' anti-GFP antibody (left panel in red) and autofluorescence (right panel in green). In this case, the cortical neuron in this unfixed thick section was first photographed for GFP autofluorescence (left), and then the section was fixed (4% paraformaldehyde) and immunostained for GFP-immunoreactivity (1:1000 dilution) using Texas Red-goat anti-chicken IgY antibodies (Jackson ImmunoResearch) as a secondary. The same cell (left) was then identified.

Immunoreactivity using anti-GFP antibody (left panel in red), Western blot showing specific immunolabeling of the GFP protein.

Immunolabeling of dividing Drosophilia larval neuroblast using Anti-GFP (green, Cat. No. GFP-1010, 1:500) to detect the GFP tagged telomere protein HOAP, and nuclei stained with DAPI (grey). Image was taken with a Zeiss 880 confocal and kindly provided by Brandt Warecki, Sullivan Lab, University of California, Santa Cruz.

Immunostaining of mouse hippocampus identifying injected HSV containing GFP (Cat GFP, 1:250, green) and DAPI (blue). Image kindly provided by Chad Brunswick, Penn State University.

Immunostaining of adult hippocampal neural stem cells from Nestin GFP trangenic mice labeling GFAP (orange, mouse) and GFP (orange, 1:1000, chicken, cat co GFP). The confocal image was obtained from a Leica Sp8 confocal microscope, magnification 40X. Image kindly provided by Soraya Martín Suarez, Achucarro Basque Center for Neuroscience, Spain.

Immunostaining of CX3CR1-Cre mouse hypothalamus showing specific detection of GFP (cat. GFP, green) and IBA1 (red). Nuclei are labeled with DAPI (blue). Image kindly provided by Karmen Ma, NIDDK – National Institutes of Health.

Immunostaining of CX3CR1-Cre mouse hypothalamus showing specific detection of GFP (cat. GFP, green) and IBA1 (red). Image kindly provided by Karmen Ma, NIDDK – National Institutes of Health.

Immunostaining of mouse primary astrocyte cultures expressing a GFP tagged GPCR, stained with GFP (cat. GFP, 1:2500). Image kindly provided by Sandeep Singh, Virginia Commonwealth University.

Representative image defining the following zebrafish microglial morphologies: ramified, cup formation, and soma retraction. White arrowheads show interactions between the microglial process (mpeg:EGFP-CAAX, Cat no GFP, green, 1:1000) and surrounding neuronal cell bodies (NBT:dsRed). Image from publication CC-BY-4.0. PMID: 38490196

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: GFP-1020

Volume: 400 µL

Variant

Price:

$500.00

Quantity:

Product Details

Target

Green Fluorescent Protein (GFP)

Target Description

Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.

Format

IgY Fraction

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Molecular Weight

27 kDa

Antigen

Recombinant GFP expressed in Escherichia coli

Antibody Registry ID

AB_2307313

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Physical State

Liquid

Production Notes

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a filter-sterilized mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL).

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product.

Shipping

Ice packs

Product Specific References for Applications and Species

FISH
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Western Blot

FISH
PMID	Dilution	Publication
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37500778	1:1000	Wang, WX, et al. 2023. Universal, label-free, single-molecule visualization of DNA origami nanodevices across biological samples using origamiFISH. Nature nanotechnology, 0.

Flow Cytometry
PMID	Dilution	Publication
34732708	1:500	Jarmas, A.E., et al. 2021. Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis. Nature Communications, 6332.

Immunocytochemistry
PMID	Dilution	Publication
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38865239	not listed	Tanaka, J, et al. 2024. Generation of salivary glands derived from pluripotent stem cells via conditional blastocyst complementation. Cell reports, 114340.
38845196	1:1000	Hanlon, KS, et al. 2024. In vivo selection in non-human primates identifies AAV capsids for on-target CSF delivery to spinal cord. Molecular therapy : the journal of the American Society of Gene Therapy, 0.
38722021	1:500	Forcella, P, et al. 2024. SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA. eLife, 0.
38754427	not listed	Dao, L, et al. 2024. Modeling blood-brain barrier formation and cerebral cavernous malformations in human PSC-derived organoids. Cell Stem Cell, .
38664376	1:500	Liu, X, et al. 2024. Numb positively regulates Hedgehog signaling at the ciliary pocket. Nature Communications, 3365.
38660387	1:500	Järvelä, V, et al. 2024. A novel pathogenic SLC12A5 missense variant in epilepsy of infancy with migrating focal seizures causes impaired KCC2 chloride extrusion. Frontiers in Molecular Neuroscience, 1372662.
38592453	1:500	Trotter, TN, et al. 2024. Overcoming Xenoantigen Immunity to Enable Cellular Tracking and Gene Regulation with Immune-competent "NoGlow" Mice. Cancer Research Communications, 1050-1062.
38574730	not listed	Irala, D, et al. 2024. Astrocyte-secreted neurocan controls inhibitory synapse formation and function. Neuron, 1657-1675.
38565145	not listed	Grommisch, D, et al. 2024. Defining the contribution of Troy-positive progenitor cells to the mouse esophageal epithelium. Developmental Cell, 1269-1283.
38454080	1:5000	Viou, L, et al. 2024. PAK3 activation promotes the tangential to radial migration switch of cortical interneurons by increasing leading process dynamics and disrupting cell polarity. Molecular Psychiatry, 0.
38442714	not listed	Krontira, AC, et al. 2024. Human cortical neurogenesis is altered via glucocorticoid-mediated regulation of ZBTB16 expression. Neuron, 0.
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38236769	1:3000	Bjornson, K.J., et al. 2024. Assessing protein distribution and dendritic spine morphology relationships using structured illumination microscopy in cultured neurons. STAR Protocols, 102829.
38177502	1:100	Bahcheli, A.T., et al. 2024. Pan-cancer ion transport signature reveals functional regulators of glioblastoma aggression. The EMBO Journal, 196-224.
37989085	1:1000	Jin, L, et al. 2023. Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency. Cell reports methods, 100644.
37969757	1:1000	Sun, XY, et al. 2023. Generation of Human Blood Vessel and Vascularized Cerebral Organoids. Bio-protocol, e4870.
37957154	1:500	Fang, TZ, et al. 2023. Knockout or inhibition of USP30 protects dopaminergic neurons in a Parkinson's disease mouse model. Nature communications, 7295.
37865312	1:500	Benner, O, et al. 2023. Multiple N-linked glycosylation sites critically modulate the synaptic abundance of Neuroligin isoforms. The Journal of Biological Chemistry, 105361.
37858465	1:1000	Hoi, KK, et al. 2023. Primary cilia control oligodendrocyte precursor cell proliferation in white matter injury via Hedgehog-independent CREB signaling. Cell Reports, 113272.
37844219	1:1000	Dingal, PCDP, et al. 2023. Molecular mechanisms controlling the biogenesis of the TGF-β signal Vg1. PNAS USA, e2307203120.
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Immunohistochemistry
PMID	Dilution	Publication
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38944834	1:2000	Henderson, F, et al. 2024. Regulation of stress-induced sleep perturbations by dorsal raphe VGLUT3 neurons in male mice. Cell reports, 114411.
38942014	1:500	Scott-Hewitt, N, et al. 2024. Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain. Cell, 0.
38936365	1:1000	Leung, BK, et al. 2024. A ventral pallidal-thalamocortical circuit mediates the cognitive control of instrumental action. Current biology : CB, 0.
38924416	1:500	Berasain, L, et al. 2024. Accelerated evolution in the human lineage led to gain and loss of transcriptional enhancers in the RBFOX1 locus. Science advances, eadl1049.
38918041	1:500	Rodríguez-Prieto, Á, et al. 2024. Nrg1 intracellular signaling regulates the development of interhemispheric callosal axons in mice. Life science alliance, 0.
38898273	1:500	Qi, L, et al. 2024. Krause corpuscles are genital vibrotactile sensors for sexual behaviours. Nature, 926-934.
38890340	1:1000	Bielefeld, P, et al. 2024. Traumatic brain injury promotes neurogenesis at the cost of astrogliogenesis in the adult hippocampus of male mice. Nature communications, 5222.
38884422	1:1000	Zhang, X, et al. 2024. The astrocyte-enriched gene deathstar plays a crucial role in the development, locomotion, and lifespan of D. melanogaster. Fly, 2368336.
38870929	not listed	Garcia-Garcia, MG, et al. 2024. A cerebellar granule cell-climbing fiber computation to learn to track long time intervals. Neuron, 0.
38870008	not listed	Vuong, LT, et al. 2024. Wg/Wnt-signaling-induced nuclear translocation of β-catenin is attenuated by a β-catenin peptide through its interference with the IFT-A complex. Cell reports, 114362.
38849524	1:1000	Foerster, S, et al. 2024. Developmental origin of oligodendrocytes determines their function in the adult brain. Nature neuroscience, 0.
38849523	1:2000	Holl, D, et al. 2024. Distinct origin and region-dependent contribution of stromal fibroblasts to fibrosis following traumatic injury in mice. Nature neuroscience, 0.
38843332	1:2000	Smith, ACW, et al. 2024. A master regulator of opioid reward in the ventral prefrontal cortex. Science (New York, N.Y.), eadn0886.
38838158	1:3000	Singh, A, et al. 2024. Gene regulatory landscape of cerebral cortex folding. Science advances, eadn1640.
38726925	1:200	Becerra Calderon, A, et al. 2024. Angiotensin II Directly Increases Endothelial Calcium and Nitric Oxide in Kidney and Brain Microvessels In Vivo With Reduced Efficacy in Hypertension. Journal of the American Heart Association, e033998.
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38778027	1:1000	Cauzzo, S, et al. 2024. A modular framework for multi-scale tissue imaging and neuronal segmentation. Nature Communications, 4102.
38772371	1:200	Wu, M, et al. 2024. Innervation of nociceptor neurons in the spleen promotes germinal center responses and humoral immunity. Cell, .
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38733583	not listed	Shrestha, H, et al. 2024. The Janus kinase 1 is critical for pancreatic cancer initiation and progression. Cell Reports, 114202.
38729764	1:1000	Zhang, X, et al. 2024. Tetrahydrofolate Attenuates Cognitive Impairment after Hemorrhagic Stroke by Promoting Hippocampal Neurogenesis via PTEN Signaling. eNeuro, .
38717903	1:1000	Donovan, EJ, et al. 2024. Dendrite architecture determines mitochondrial distribution patterns in vivo. Cell Reports, 114190.
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38710338	1:1000	Fioriti, F, et al. 2024. Bacterial peptidoglycan serves as a critical modulator of the gut-immune-brain axis in Drosophila. Brain, Behavior, and Immunity, 878-897.
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38678557	1:1000	Buchan, MJ, et al. 2024. Higher-order thalamocortical circuits are specified by embryonic cortical progenitor types in the mouse brain. Cell Reports, 114157.
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38657057	1:1000	Rein, B, et al. 2024. MDMA enhances empathy-like behaviors in mice via 5-HT release in the nucleus accumbens. Science Advances, eadl6554.
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38607918	1:1000	Tamboli, S, et al. 2024. Mouse hippocampal CA1 VIP interneurons detect novelty in the environment and support recognition memory. Cell Reports, 114115.
38607913	1:200	Sun, J, et al. 2024. ptx3a+ fibroblast/epicardial cells provide a transient macrophage niche to promote heart regeneration. Cell Reports, 114092.
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38569472	1:2000	Guan, D, et al. 2024. Central inhibition of HDAC6 re-sensitizes leptin signaling during obesity to induce profound weight loss. Cell Metabolism, 857-876.e10.
38553047	1:1000	Weman, HM, et al. 2024. Spinal glycine receptor alpha 3 cells communicate sensations of chemical itch in hairy skin. The Journal of Neuroscience, 0.
38548715	1:500	Nagy, GN, et al. 2024. Structure and function of Semaphorin-5A glycosaminoglycan interactions. Nature Communications, 2723.
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38459070	1:4000	Virga, DM, et al. 2024. Activity-dependent compartmentalization of dendritic mitochondria morphology through local regulation of fusion-fission balance in neurons in vivo. Nature Communications, 2142.
38454052	1:750	Santos, TB, et al. 2024. Reactivation of encoding ensembles in the prelimbic cortex supports temporal associations. Neuropsychopharmacology, 0.
38448588	5ug/ml	Schappe, MS, et al. 2024. A vagal reflex evoked by airway closure. Nature, 830-838.
38447890	1:3000	Shibayama, K, et al. 2024. The serotonergic neurons derived from rhombomere 2 are localized in the median raphe and project to the dorsal pallium in zebrafish. Neuroscience Research, 0.
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38442711	1:500	Qi, L, et al. 2024. A mouse DRG genetic toolkit reveals morphological and physiological diversity of somatosensory neuron subtypes. Cell, 1508-1526.e16.
38429929	1:1000	Schuster, KH, et al. 2024. ASOs are an effective treatment for disease-associated oligodendrocyte signatures in premanifest and symptomatic SCA3 mice. Molecular Therapy, 0.
38418648	1:1000	Wang, M, et al. 2024. Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain. Nature Biotechnology, 0.
38431221	1:200	De Bortoli, M, et al. 2024. Somatic loss-of-function PIK3R1 and activating non-hotspot PIK3CA mutations associated with Capillary Malformation with Dilated Veins (CMDV). The Journal of investigative dermatology, .
38421871	1:1000	Chen, H, et al. 2024. The functional and anatomical characterization of three spinal output pathways of the anterolateral tract. Cell reports, 113829.
38412857	1:500	Wang, C, et al. 2024. Impaired cerebellar plasticity hypersensitizes sensory reflexes in SCN2A-associated ASD. Neuron, .
38386556	1:1000	Wang, H, et al. 2024. Parallel pathways carrying direction-and orientation-selective retinal signals to layer 4 of the mouse visual cortex. Cell reports, 113830.
38386552	1:500	Connell, M, et al. 2024. Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression. Cell reports, 113823.
38381829	1:500	Dong, Y, et al. 2024. Single-cell chromatin profiling reveals genetic programs activating proregenerative states in nonmyocyte cells. Science advances, eadk4694.
38378693	1:500	Oudhoff, H, et al. 2024. Skeletal muscle regeneration after extensive cryoinjury of caudal myomeres in adult zebrafish. NPJ Regenerative medicine, 8.
38367510	1:1000	Zhang, X, et al. 2024. Iron/ROS/Itga3 mediated accelerated depletion of hippocampal neural stem cell pool contributes to cognitive impairment after hemorrhagic stroke. Redox biology, 103086.
38363046	1:500	Mercier, O, et al. 2024. Transient demyelination causes long-term cognitive impairment, myelin alteration and network synchrony defects. Glia, 960-981.
38355299	1:6000	Janowitz, HN, et al. 2024. Chronic Treatment with Serotonin Selective Reuptake Inhibitors Does Not Affect Regrowth of Serotonin Axons Following Amphetamine Injury in the Mouse Forebrain. eNeuro, .
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Western Blot
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Anti-Green Fluorescent Protein Antibody

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