Immunoblot against extracts of COS cells transiently transfected with untagged GlyRAlpha3L, GlyRAlpha3K or Kv2.1 plasmid probed with N424/45 (left) or K89/34 (right) TC supe.
Anti-Glycine Receptor Alpha3 Antibody
Glycine receptor subunit alpha-3
Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).
Fusion protein amino acids 342-431 (cytoplasmic loop) of human GlyRAlpha3L (also known as Glycine receptor subunit alpha-3 and GLRA3, accession number O75311); Mouse: 95% identity (86/90 amino acids identical); Rat: 93% identity (84/90 amino acids identical)80% identity with GlyRAlpha3K (amino acids 358-372 or TEAFALEKFYRFSDM is L-specific); ~50% identity with GlyRAlpha1 and GlyRAlpha2
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Cross-reacts with GlyRAlpha3K
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.