Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

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Our Anti-Glial Fibrillary Acidic Protein (GFAP) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects bovine, horse, human, mouse, and rat Glial Fibrillary Acidic Proetin (GFAP) and is neat serum. It is great for use in WB, IHC, ICC.

Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.
Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (cat. 620-GFAP, 1:5000,green), colabeled with anti-MeCP2 (cat. 1205-MeCP2, 1:500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.
Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (cat. 620-GFAP, 1:5000,green), colabeled with anti-calbindin ( cat. 302-CALB , 1:2,000, red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.
Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (cat. 1451-NFH, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP (cat. 620-GFAP, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
Mixed neuron/glial cultures stained with anti-vimentin (green, 1:500) and rabbit anti-GFAP antibody (cat. 620-GFAP, red, 1:1000). The blue stains nuclear DNA. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
Immunofluorescence of a section of rat cerebellum labeled with anti-visinin-like 1 protein (cat. 2145-VSNL1, 1:500, red) and anti-GFAP (cat. 620-GFAP, green, 1:5000). The anti-VSNL1 revels protein expressed in the granule cell menbranes and in synapses in the white matter and molecular layers. The anti-GFAP reveals protein expressed in the processes of Bergmann glia and astroglia.
Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.
Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (cat. 620-GFAP, 1:5000,green), colabeled with anti-MeCP2 (cat. 1205-MeCP2, 1:500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.
Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (cat. 620-GFAP, 1:5000,green), colabeled with anti-calbindin ( cat. 302-CALB , 1:2,000, red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.
Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (cat. 1451-NFH, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP (cat. 620-GFAP, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
Mixed neuron/glial cultures stained with anti-vimentin (green, 1:500) and rabbit anti-GFAP antibody (cat. 620-GFAP, red, 1:1000). The blue stains nuclear DNA. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
Immunofluorescence of a section of rat cerebellum labeled with anti-visinin-like 1 protein (cat. 2145-VSNL1, 1:500, red) and anti-GFAP (cat. 620-GFAP, green, 1:5000). The anti-VSNL1 revels protein expressed in the granule cell menbranes and in synapses in the white matter and molecular layers. The anti-GFAP reveals protein expressed in the processes of Bergmann glia and astroglia.

Rat
ICC, IHC, WB
Rabbit

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Data Sheet
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SKU: 620-GFAP

Volume: 100 µL
Price:
$375.00

Product Specific References for Applications and Species

  • Immunohistochemistry: Mouse | Rat
  • Western Blotting: Rat
Immunohistochemistry: Mouse
PMID Dilution Publication
33675359 1:300 Yu, D., et al. 2021. The Transcription Factor Shox2 Shapes Neuron Firing Properties and Suppresses Seizures by Regulation of Key Ion Channels in Thalamocortical Neurons. Cerebral Cortex.
31875545 1:300 Chen, C., et al. 2019. Astrocytes amplify neuronal dendritic volume transmission stimulated by norepinephrine. Cell Reports, 29(13), pp.4349-4361.
Immunohistochemistry: Rat
PMID Dilution Publication
35406701 1:1000 Haggert, A.E., et al. 2022. The Effects of the Combination of Mesenchymal Stromal Cells and Nanofiber-Hydrogel Composite on Repair of the Contused Spinal Cord. Cells, 11(7), p.1137.
Western Blotting: Rat
PMID Dilution Publication
29916754 1:1000 Homiack, D., et al. 2018. Effect of acute alarm odor exposure and biological sex on generalized avoidance and glutamatergic signaling in the hippocampus of Wistar rats. Stress, Jul;21(4):292-303.

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