Adult WT and KO mouse hippocampus immunofluorescence with N206A/8 (green) and KC rabbit anti- Kv2.1 (red). Brain samples courtesy of Albee Messing, University of Wisconsin at Madison.
Glial fibrillary acidic protein (GFAP) is an intermediate filament with many different isoforms, and acts as a structural protein in the cytoskeleton. It is expressed in astrocytes in brain and is often used as an astrocyte marker. GFAP is belived to be involved in many CNS processes including cell communication and mitosis. GFAP has been found to be involved in Alexander disease and Wernicke's encephalopathy.
ICC, IHC, WB
Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (also known as Glial fibrillary acidic protein, accession number P14136); Rat: 100% identity (12/12 amino acids identical); Mouse: 100% identity (12/12 amino acids identical)
Greater than 50% identity with other proteins (Vimentin, Desmin, Tumor protein p73 and Peripherin)
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Cross-reacts with GFAP-R416W and other GFAP mutant proteinsDoes not cross-react with other proteins (based on KO validation results)
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.