Anti-GABAA Receptor δ, N-Terminus Antibody

Name: Anti-GABAA Receptor δ, N-Terminus Antibody
Brand: PhosphoSolutions
SKU: 868A-GDN
Price: 415.0 USD
Availability: InStock

from PhosphoSolutions Datasheet Trial Size Pooled Serum

3 Citations

Our Anti-GABAA Receptor δ, N-Terminus primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat GABAA Receptor δ, N-Terminus and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GABRD

Molecular Weight

50 kDa

Go to Full Product Details

Western blot of mouse whole brain (1) and mouse synaptic plasma membrane (2) lysates showing specific immunolabeling of the ~50 kDa δ-subunit of the GABAA-R.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-GABAA Receptor δ, N-Terminus Antibody

Product Details

Target

GABAA Receptor δ, N-Terminus

Target Description

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl– channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin,1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, co-expression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). More recently there have been a number of studies demonstrating that the δ-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

GABRD

Molecular Weight

50 kDa

Antigen

Fusion protein from the N-terminus of the δ subunit of rat GABAA receptor.

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2631037

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via chromatography on fusion protein immunogen column.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:100
ICC: 1:500**

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~50 kDa δ-subunit of the GABAA receptor.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

GABA(A) receptor subunit delta antibody, Gabrd antibody, Gamma aminobutyric acid GABA A receptor delta antibody, Gamma aminobutyric acid receptor delta subunit precursor GABA A receptor antibody, Gamma-aminobutyric acid receptor subunit delta antibody, GBRD_HUMAN antibody, MGC45284 antibody

UniProt Details

UniProt (Immunogen Species): P18506

NCBI Gene ID

29689

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Electon Microscopy (PostEmbedding ImmunoGold): Mouse
Western Blot: Rat

Immunohistochemistry: Mouse
PMID	Dilution	Publication
32041742	1:100	Agoglia, A.E., et al. 2021. Corticotropin-Releasing Factor Receptor-1 Neurons in the Lateral Amygdala Display Selective Sensitivity to Acute and Chronic Ethanol Exposure. Eneuro, 7(2).

PostEmbedding ImmunoGold Labeling EM: Mouse
PMID	Dilution	Publication
35934131	1:100	Sun, Y., et al. 2022. Virally-Induced Expression of GABAA Receptor δ Subunits Following Their Pathological Loss Reveals Their Role in Regulating GABAA Receptor Assembly. Progress in Neurobiology, 102337.

Western Blot: Rat
PMID	Dilution	Publication
35320456	1:500	Sancho-Alonso, M., et al. 2022. Hyperammonemia Enhances GABAergic Neurotransmission in Hippocampus: Underlying Mechanisms and Modulation by Extracellular cGMP. Molecular Neurobiology.

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Anti-GABAA Receptor δ, N-Terminus Antibody

Our Anti-GABAA Receptor δ, N-Terminus primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat GABAA Receptor δ, N-Terminus and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.