Our Anti-GABAA Receptor α1, N-Terminus primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat GABAA Receptor α1, N-Terminus and is antigen affinity purified. It is great for use in WB, IHC.
Western blot of mouse forebrain lysates from Wild Type (WT) and α1-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α1-knockout animals.
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Antigen Affinity Purified
Polyclonal
IgG
IHC, WB
Rabbit
GABRA1
51 kDa
Synthetic peptide corresponding to amino acid residues from the N-terminal region of the α1 subunit of rat GABAA, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Canine, Human, Non-Human Primate
AB_2492100
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:100
Unconjugated
Specific for endogenous levels of the ~51 kDa α1-subunit of the GABAA receptor. Immunolabeling is absent in α1-subunit knockout animals.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Agoglia, A.E., et al. 2020. Corticotropin-Releasing Factor Receptor-1 Neurons in the Lateral Amygdala Display Selective Sensitivity to Acute and Chronic Ethanol Exposure. Eneuro, 7(2).
Herman, M., et al. 2016. Cell‐type‐specific tonic GABA signaling in the rat central amygdala is selectively altered by acute and chronic ethanol. Addiction biology. Jan;21(1):72-86.
Engin, E., et al. 2015. Tonic Inhibitory Control of Dentate Gyrus Granule Cells by α5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712.
Kurano, Y., et al. 2006. . Chorein deficiency leads to upregulation of gephyrin and GABAA receptor. Biochemical and biophysical research communications, 351(2), pp.438-442.
