Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).
Gamma-Aminobutyric Acid Type A Receptor Alpha5 Subunit or GABA(A) Receptor, Alpha 5 is encoded by the gene GABRA5. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 5 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR alpha5 is highly expressed in brain at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. Disorders involving the GABRA5 gene include Angelman Syndrome, Prader-willi syndrome and childhood absence epilepsy.
ICC, IHC, WB
Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA-A-R-Alpha5 (also known as Gamma-aminobutyric acid receptor subunit alpha-5, GABA(A) receptor subunit alpha-5 and Gabra5, accession number P31644); Mouse: 78% identity (41/52 amino acids identical); Rat: 73% identity (39/53 amino acids identical); 50% identity with GABA-A-R-Alpha2 and other GABA-A receptors
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