Anti-GABA-A-R-Alpha5 Antibody (N415/24)

Name: Anti-GABA-A-R-Alpha5 Antibody
Brand: NeuroMab
SKU: 75-401
Price: 337.0 USD
Availability: InStock

Our mouse GABA-A-R-Alpha5 monoclonal antibody from NeuroMab is produced in-house from clone N415/24. It detects human, mouse, and rat GABA-A-R-Alpha5 and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, ELISA.

$Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).$

Immunolabelling showing α1-/α5-containing GABAA receptors demonstrated synaptic localizations in a primary hippocampal neuronal culture. Distributions of α1 and α5 (Cat. 75-401, 1:1000) subunits (A,B) were co-stained with the inhibitory presynaptic marker protein vGat (C). (D) The α1 subunit was mostly synaptically localized, opposing vGat, whereas α5 was distributed at both the soma and synapse and often showed co-localization with α1-vGat puncta at the synapse (E,F). CC-BY-4.0. PMID:38201218

Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-401

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GABA-A-R-Alpha5

Scientific Background

Gamma-Aminobutyric Acid Type A Receptor Alpha5 Subunit or GABA(A) Receptor, Alpha 5 is encoded by the gene GABRA5. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 5 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR alpha5 is highly expressed in brain at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. Disorders involving the GABRA5 gene include Angelman Syndrome, Prader-willi syndrome and childhood absence epilepsy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N415/24

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

GABRA5

Molecular Weight

55 kDa

Immunogen

Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA-A-R-Alpha5 (accession number P31644) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2491187

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with GABA-A-R-Alpha2 (or other GABAA

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

GAα5; Gamma-aminobutyric acid receptor subunit alpha-5; GAA5; GABA(A receptor subunit alpha-5; GABA(A receptor subunit alpha-5; GABRα5; Gabra5; Gamma aminobutyric acid GABA A receptor alphα5; Gamma aminobutyric acid GABA A receptor alphα5 precursor; Gamma aminobutyric acid receptor alphα5 subunit precursor GABA A receptor; Gamma-aminobutyric acid receptor subunit alpha-5; GBRA5_HUMAN; GC138184

UniProt ID

P31644
UniProt Summary: Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. GABAARs containing alpha-5/GABRA5 subunits are mainly extrasynaptic and contribute to the tonic GABAergic inhibition in the hippocampus. Extrasynaptic alpha-5-containing GABAARs in CA1 pyramidal neurons play a role in learning and memory processes.

NCBI Gene ID

2558

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Shipped on ice packs

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Anti-GABA-A-R-Alpha5 Antibody (N415/24)

SKU: 75-401

Product Details

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