Anti-Eukaryotic translation initiation factor 2C (elF2c) Antibody

Name: Anti-Eukaryotic translation initiation factor 2C (elF2c) Antibody
Brand: Biosensis
SKU: R-1556-100
Price: 337.0 USD
Availability: InStock

Our Anti-Eukaryotic translation initiation factor 2C (elF2c) rabbit polyclonal primary antibody detects human, mouse, and rat Eukaryotic translation initiation factor 2C (elF2c), and is affinity-purified. It is validated for use in IHC-Frozen, IHC-Paraffin-embedded, IP, WB.

Immunohistochemical staining with Rabbit polyclonal antibody to mouse elF2c (R-1556-100) in(B) mouse hippocampal area. Fixed (4% paraformaldehyde) mouse brain free floating sections were incubated with R-1556-100 at 1 µg/mL overnight followed by incubation with fluoroscein donkey anti-rabbit IgG conjugate at a dilution of 1:100. Scale bar = 20µm.

Immunohistochemical staining with Rabbit polyclonal antibody to mouse elF2c (R-1556-100) in (D) mouse cerebellar cortex. Fixed (4% paraformaldehyde) mouse brain free floating sections were incubated with R-1556-100 at 1 µg/mL overnight followed by incubation with fluoroscein donkey anti-rabbit IgG conjugate at a dilution of 1:100. Scale bar = 20µm.

Western Blot in mouse brain extracts using Rabbit polyclonal to mouse elF2c protein (R-1556-100). Figure A: Total mouse brain extract. Detection at 0.03 µg/mL. Protein loaded 20 µg. Figure B: Mouse cytosolic brain extract. Detection at 0.03 µg/mL. Protein loaded 20 µg, 10 µg and 5 µg.

Immunohistochemical staining with Rabbit polyclonal antibody to mouse elF2c (R-1556-100) in(B) mouse hippocampal area. Fixed (4% paraformaldehyde) mouse brain free floating sections were incubated with R-1556-100 at 1 µg/mL overnight followed by incubation with fluoroscein donkey anti-rabbit IgG conjugate at a dilution of 1:100. Scale bar = 20µm.

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Species Reactivity

Human, Mouse, Rat

Applications

EM, IHC, IP, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1556-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Product Details

Target

Eukaryotic translation initiation factor 2C (elF2c)

Scientific Background

The elF2c (Argonaute, Ago) protein is a core protein of the RNA-induced silencing complex (RISC).

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

EM, IHC, IP, WB

Host Species

Rabbit

Molecular Weight

94 kDa

Immunogen

A synthetic peptide corresponding to a sequence (845-860 aa) from the C-terminus of mouse elF2C conjugated to KLH.

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized from PBS.

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:200-1:500

Conjugate

Unconjugated

Specificity

Specificity has been confirmed by IHC, WB and IP in mouse brain. This antibody is expected to recognize at least elF2c-1, 2 and 3 since they have identical C-termini. Human; mouse; rat; The Ago proteins are highly conserved so cross-reactivity with other species is expected.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Protein argonaute-1; Protein argonaute-2; Protein argonaute-3; Argonaute1; Argonaute2; Argonaute3; mAgo1; mAgo2; Eukaryotic translation initiation factor 2C; eIF-2C 2; eIF-2C 1; eIF2C 2; Piwi/argonaute family protein meIF2C2 Protein slicer; Eif2c2; Ago1; Ago2;

UniProt ID

Q8CJG0

Shipping

25°C (ambient)

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Anti-Eukaryotic translation initiation factor 2C (elF2c) Antibody

SKU: R-1556-100

Product Details

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