Name: Anti-Doublecortin Antibody
Brand: Aves Labs
SKU: DCX
Price: 410.0 USD
Availability: InStock

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (AAT58219.1), rat (NP_445831.3) and human (CAA06617.1) gene products. These antibodies were affinity-purified and then mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Clonality

Polyclonal

Applications

ICC, IHC

Host Species

Chicken

Gene Name

DCX DBCN LISX

Go to Full Product Details

Doublecortin (DCX, Aves Labs, 1:1000 dilution) staining (GREEN) of neuroblastoma cells in culture. RED staining is a Golgi apparatus marker. Page Balisch (University of Arizona)

Doublecortin (DCX, Aves Labs, 1:1000 dilution) staining (GREEN) of a tissue section (4% paraformaldehyde-fixed, paraffin-embedded) through the cochlear ganglion from a neonatal mouse. RED staining is neurofilament [NF-M, Rockland Labs, visualized with Texas Red-goat anti-rabbit IgG (Jackson Immunoresearch)]

Doublecortin (DCX, Aves Labs, 1:1000 dilution) staining (GREEN) of a tissue section (4% paraformaldehyde-fixed, paraffin-embedded) through the cerebellar cortex of a neonatal mouse. REF staining is neurofilament [NF-M, Rockland Labs, visualized with Texas Red-goat anti-rabbit IgG (Jackson Immunoresearch)]

Doublecortin (DCX, Aves Labs, 1:1000 dilution) staining (RED) of a tissue section (4% paraformaldehyde-fixed, paraffin-embedded) through the cerebral cortical periventricular zone cerebellar cortex of a neonatal mouse. Green staining is neurofilament [NF-M, Rockland Labs, visualized with fluorescein-goat anti-rabbit IgG (Jackson Immunoresearch)]

Doublecortin (DCX, Aves Labs, 1:1000 dilution) staining (GREEN) of neuroblastoma cells in culture. RED staining is a Golgi apparatus marker. Page Balisch (University of Arizona)

Product Details

Target

Doublecortin

Target Description

Doublecortin is a microtubule-associated protein found in neuroblasts and immature neurons, especially those undergoing embryonic migrations. It was first identified as the X-chromosome-encoded gene product mutated in the human diseases Lissencephaly (in males) and Periventricular Heterotopia (in females), diseases caused by failure of neocortical neuroblast migration. The human protein itself is 360 amino acids (40,044 daltons) and contains doublecortin motifs near its N-terminus that are believed to mediate the microtubule binding.

Concentration

200 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

DCX DBCN LISX

Antigen

Synthetic peptide

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313540

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the doublecortin gene product, but are shared between the human (CAA06617.1, NCBI) and mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of these affinity-purified antipeptide antibodies were mixed, and the preparation was filter-sterilized.

Dilution Ranges

IHC: 1:1000-1:2000

Quality Control

This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

Ice packs

Synonyms

Neuronal migration protein doublecortin (Doublin) (Lissencephalin-X) (Lis-X)

UniProt Details

UniProt (Immunogen Species): O15075

Citations (4)

Smagin DA, Park JH, Michurina TV, Peunova N, Glass Z, Sayed K, Bondar NP, Kovalenko IN, Kudryavtseva NN, Enikolopov G (2015), 'Altered Hippocampal Neurogenesis and Amygdalar Neuronal Activity in Adult Mice with Repeated Experience of Aggression.' Frontiers in Neurosciene. 10.3389/fnins.2015.00443.
Lim RG, Salazar LL, Wilton DK, King AR, Stocksdale JT, Sharifabad D, Lau AL, Stevens B, Reidling JC, Winokur ST, Casale MS, Thompson LM, Pardo M, Díaz-Barriga AGG, Straccia M, Sanders P, Alberch J, Canals JM, Kaye JA, Dunlap M, Jo L, May H, Mount E, Anderson-Bergman C, Haston K, Finkbeiner S, Kedaigle AJ, Gipson TA, Yildirim F, Ng CW, Milani P, Housman DE, Fraenkel E, Allen ND, Kemp PJ, Atwal RS, Biagioli M, Gusella JF, MacDonald ME, Akimov SS, Arbez N, Stewart J, Ross CA, Mattis VB, Tom CM, Ornelas L, Sahabian A, Lenaeus L, Mandefro B, Sareen D, Svendsen CN (2017), 'Developmental alterations in Huntington's disease neural cells and pharmacological rescue in cells and mice.' Nature Neuroscience. 10.1038/nn.4532.
Mellios N, Feldman DA, Sheridan SD, Ip JPK, Kwok S, Amoah SK, Rosen B, Rodriguez BA, Crawford B, Swaminathan R, Chou S, Li Y, Ziats M, Ernst C, Jaenisch R, Haggarty SJ, Sur M (2017), 'MeCP2-regulated miRNAs control early human neurogenesis through differential effects on ERK and AKT signaling.' Molecular Psychiatry. 10.1038/mp.2017.86.
Mehta SR, Tom CM, Wang Y, Bresee C, Rushton D, Mathkar PP, Tang J, Mattis VB (2018), 'Human Huntington’s Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation.' Cell Reports. 10.1016/j.celrep.2018.09.076.
A Alachkar, L Wang, R Yoshimura, AR Hamzeh, Z Wang, N Sanathara, SM Lee, X Xu, GW Abbott, and O Civelli (2017), 'Prenatal one-carbon metabolism dysregulation programs schizophrenia-like deficits.' Molecular Psychiatry. 10.1038/mp.2017.164.

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Anti-Doublecortin Antibody