Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged CDY1, HA- and Flag- tagged CDYL1 and CDYL2 and untagged Kv2.1 plasmids and probed with N143/38 (left panel), anti-Flag (middle panel) and K89/34 (right panel) TC supe.
This antibody recognizes the proteins Testis-specific chromodomain protein Y 1 and 2 and are encoded by the genes CDY1 and CDY2A respectively. These genes each have a paralog (CDY1B and CDY2B) which are also present on the Y chromosome. Proteins in the CDY-related family contain a chromodomain, involved in chromatin binding, and a histone acetyltransferase catalytic domain. These proteins are expressed in the nucleaus in testis. Diseases associated with these genes include Y-Linked Spermatogenic Failure, and Y Chromosome Infertility.
CDY1 CDY1A; CDY1B
Fusion protein amino acids 6-66 (Chromo domain) of human CDY1 (also known as Testis-specific chromodomain protein Y 1, accession number Q9Y6F8)
100% identity with human CDY273% identity with human CDYL1 (44/60 amino acids identical)
51% identity with human CDYL2 (31/60 amino acids identical)
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.