Anti-Catenin beta Antibody

Name: Anti-Catenin beta Antibody
Brand: Biosensis
SKU: S-1233-100
Price: 387.0 USD
Availability: InStock

Our Anti-Catenin beta sheep polyclonal primary antibody detects human, mouse, and rat Catenin beta, and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.

Western blot analysis of Catenin beta (S-1233-100) in extracts from HepG2 cells: untreated (Lane 1) and treated with GSK3 inhibitor (Lane 2).

Staining at cell junctions of active Catenin beta in INS-E cells in culture using Sheep polyclonal antibody to dephosphorylated Catenin beta S-1233-100.

Western blot analysis of Catenin beta (S-1233-100) in extracts from HepG2 cells: untreated (Lane 1) and treated with GSK3 inhibitor (Lane 2).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Sheep

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Data Sheet

SKU: S-1233-100

Volume/Size: 100 µg

Variant

Price:

$387.00

Quantity:

Product Details

Target

Catenin beta

Scientific Background

Catenin beta is an adherens junction protein and has a role in the regulation of cell adhesion and in signal transduction through the Wnt pathway. At least 2 isoforms are produced by alternative splicing.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Sheep

Molecular Weight

86-92 kDa

Immunogen

A synthetic peptide (C-QSYLDSGIHSGATTTAPSL) corresponding to the amino acid region 28-46 of human Catenin beta coupled to carrier protein KLH. This antibody was designed to detect the active form of Catenin beta . The antibody exhibits a strong preference for dephosphorylated beta catenin protein by western blot but the antibody may show cross reactivity with phosphorylated catenin beta under certain conditions.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized from 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, and 0.05 % sodium azide.

Dilution Ranges

WB: 2 µg/mL
ICC: 4 µg/mL

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; _-Catenin;

UniProt ID

P35222

Shipping

25°C (ambient)

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Anti-Catenin beta Antibody

SKU: S-1233-100

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