Anti-Axl/UFO (Extracellular region) Antibody (M047)

Name: Anti-Axl/UFO (Extracellular region) Antibody
Brand: PhosphoSolutions
SKU: AM0471
Price: 370.0 USD
Availability: InStock

Our Axl/UFO (Extracellular region) Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Axl/UFO (Extracellular region) Antibody and is Protein G purified. It is great for use in WB, ICC, IP.

Western blot of human MDA-MB-231 breast carcinoma (lane 1), PC-3 prostate adenocarcinoma (lane 2), A549 lung carcinoma (lane 3), NCI-H1915 lung carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-Axl (AM0471) at 1:1000.

Immunocytochemical labeling of Axl in aldehyde fixed human MDAMB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Axl (AM0471). The antibody was detected using goat anti-mouse DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-Axl (AM0471) for ELISA capture of human recombinant Axl extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.

Western blot of human MDA-MB-231 breast carcinoma (lane 1), PC-3 prostate adenocarcinoma (lane 2), A549 lung carcinoma (lane 3), NCI-H1915 lung carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-Axl (AM0471) at 1:1000.

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Species Reactivity

Human

Applications

ICC, IP, WB

Host Species

Mouse

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Data Sheet

SKU: AM0471

Volume: 100 µL

Variant

Price:

$370.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Axl/UFO (Extracellular region) Antibody

Scientific Background

The Axl/UFO receptor tyrosine kinase (RTKs) family includes Axl/UFO/Tyro7, Sky/Tyro3, and c-Mer/Tyro12. These RTKs have a conserved intracellular tyrosine kinase domain and extracellular domains that include immunoglobulin-like and fibronectin-type moieties similar to those found in cell adhesion molecules. The ligand for these receptors is the vitamin Kdependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor. Upon binding to its receptor, Gas6 activates phosphatidylinositol 3- kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB. Axl is overexpressed in several cancers, including breast, lung, liver, colon, gastric, ovarian, pancreatic, and glioblastoma. The Axl/Gas6 signalling pathway has been shown to drive cancer cell survival, proliferation, migration and invasion, and several therapeutic strategies are being developed to regulate Axl cell signaling.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M047

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

AXL

Molecular Weight

140

Immunogen

Clone (M047) was generated from a recombinant protein that included the extracellular region of human Axl protein.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

Clone M047 mouse monoclonal antibody detects a 140 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human A549, NCI-H1915, and MDA-MB-231 carcinomas. The antibody detects Axl in membranes and cytoplasm in MDA-MB-231 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Tyrosine-protein kinase receptor, UFO, Axl, Tyro7, ARK,

UniProt ID

P30530

Shipping

Blue Ice

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Anti-Axl/UFO (Extracellular region) Antibody (M047)

SKU: AM0471

Product Details

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