Our Anti-Aquaporin 4 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse and rat Aquaporin 4 and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat whole brain lysate showing specific immunolabeling of the ~35 kDa AQP4 protein.
Aquaporin-4 (AQP4), a bidirectional water channel protein, is the most expressed aquaporin within the central nervous system. AQP4 is predominantly expressed by astrocytes and ependymal cells within the blood-brain-barrier and ependymal-cerebrospinal fluid barriers (Verkman, et al 2011). AQP4 plays a role in synaptic plasticity (Skucas et al, 2011), astrocyte mitigation (Saadoun et al, 2005), and K+ homeostasis (Binder et al, 2006). Due to the significant role AQP4 plays in cognition, it has been reported to be dysregulated in several neurological disorders. Alzheimer’s patients have amyloid deposits in the walls of the vasculature known as CAA which causes AQP4 mis-location (Wilcock et al, 2009). Patients with Parkinson’s disease have low levels of AQP4 expression which leads to reduced inflammatory response (Chi et al,2011). Reduced levels of AQP4 in traumatic brain injury affects both the acute stage, decreasing the ability to remove excess water from the brain, and in the later stage, by preventing cellular damage and swelling (Zhang et al, 2015).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, WB
Rabbit
AQP4
35 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat AQP4, conjugated to keyhole limpet hemocyanin (KLH).
Rat
Human, Mouse, Rat
AB_2890667
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column prepared with the C-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~35 kDa aquaporin 4 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
AQP 4; AQP-4; AQP4; AQP4_HUMAN; Aquaporin type 4; Aquaporin-4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; Mercurial-insensitive water channel; MGC22454; MIWC; WCH 4; WCH4
P47863
UniProt Summary: Forms a water-specific channel (PubMed:19383790, PubMed:7559426, PubMed:8601457). Plays an important role in brain water homeostasis (PubMed:37143309). It is involved in glymphatic solute transport and is required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity). {ECO:0000250|UniProtKB:P55088, ECO:0000269|PubMed:19383790, ECO:0000269|PubMed:37143309, ECO:0000269|PubMed:7559426, ECO:0000269|PubMed:8601457}.
Verkman, A.S., 2011. Aquaporins at a glance. Journal of Cell Science, 124(13), pp.2107-2112.
Skucas, V.A., Mathews, I.B., Yang, J., Cheng, Q., Treister, A., Duffy, A.M., Verkman, A.S., Hempstead, B.L., Wood, M.A., Binder, D.K. and Scharfman, H.E., 2011. Impairment of select forms of spatial memory and neurotrophin-dependent synaptic plasticity by deletion of glial aquaporin-4. Journal of Neuroscience, 31(17), pp.6392-6397.
Saadoun, S., Papadopoulos, M.C., Watanabe, H., Yan, D., Manley, G.T. and Verkman, A.S., 2005. Involvement of aquaporin-4 in astroglial cell migration and glial scar formation. Journal of Cell Science, 118(24), pp.5691-5698.
Binder, D.K., Yao, X., Zador, Z., Sick, T.J., Verkman, A.S. and Manley, G.T., 2006. Increased seizure duration and slowed potassium kinetics in mice lacking aquaporin‐4 water channels. Glia, 53(6), pp.631-636.
Wilcock, D.M., Vitek, M.P. and Colton, C.A., 2009. Vascular amyloid alters astrocytic water and potassium channels in mouse models and humans with Alzheimer’s disease. Neuroscience, 159(3), pp.1055-1069.
Chi, Y., Fan, Y., He, L., Liu, W., Wen, X., Zhou, S., Wang, X., Zhang, C., Kong, H., Sonoda, L. and Tripathi, P., 2011. Novel role of aquaporin‐4 in CD4+ CD25+ T regulatory cell development and severity of Parkinson’s disease. Aging Cell, 10(3), pp.368-382.
Zhang, C., Chen, J. and Lu, H., 2015. Expression of aquaporin-4 and pathological characteristics of brain injury in a rat model of traumatic brain injury. Molecular Medicine Reports, 12(5), pp.7351-7357.
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