Anti-Ankyrin-B Antibody (N105/13)

Name: Anti-Ankyrin-B Antibody
Brand: NeuroMab
SKU: 75-144
Price: 385.0 USD
Availability: InStock

Our Anti-Ankyrin-B mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N105/13. It detects human, mouse, and rat Ankyrin-B, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

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Species Reactivity

Bovine, Guinea Pig, Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-144

Volume: 100 µL

Variant

Price:

$385.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ankyrin-B

Target Description

Ankyrin-2 or -Ankyrin-B is encoded by the gene ANK2. Ankyrin B is a scaffold protein that functions to link proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane cytoskeleton. In this manner, ankyrin family proteins play key roles in various processes including cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin B is expressed in brain and muscle. Mutations in this gene cause long QT syndrome 4 and cardiac arrhythmia syndrome.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N105/13

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

ANK2 ANKB

Molecular Weight

>200 kDa

Antigen

Fusion protein amino acids 203-496 of human Ankyrin-B (accession number Q01484) produced recombinantly in E. Coli

Species Reactivity

Bovine, Guinea Pig, Human, Mouse, Rat

Antibody Registry ID

AB_10673094

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.70

Dilution Ranges

WB: 1:500
IHC: 1:500
ICC: 1:500
IP: 3 ug

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Ankyrin-2 (ANK-2) (Ankyrin-B) (Brain ankyrin) (Non-erythroid ankyrin)

UniProt ID

Q01484

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse
Immunohistochemistry: Guinea Pig | Rat
Immunoprecipitation: Bovine | Mouse
Western Blot: Bovine | Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
27001749	1:500	Patzke, C., et al. 2016. Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation.. Journal of Experimental Medicine, 499-515.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
28103480	not listed	Liu, Y., et al. 2017. Adrenergic gate release for spike timing-dependent synaptic potentiation. Neuron, 394-408.
25362473	not listed	Ho, T.S., et al. 2015. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier.. Nature Neuroscience, 1664-72.
25362471	not listed	Chang, K.J., et al. 2014. Glial ankyrins facilitate paranodal axoglial junction assembly. Nature Neuroscience, 1673-1681.

Immunohistochemistry: Guinea Pig
PMID	Dilution	Publication
21390223	1:100	Huff, T.B., et al. 2011. Real-time CARS imaging reveals a calpain-dependent pathway for paranodal myelin retraction during high-frequency stimulation.. PLoS One, e17176.

Immunohistochemistry: Rat
PMID	Dilution	Publication
21734302	1:2000	Lysakowski, A., et al. 2011. Molecular microdomains in a sensory terminal, the vestibular calyx ending.. Journal of Neuroscience, 10101-10114.

Immunoprecipitation: Bovine
PMID	Dilution	Publication
26373354	not listed	Laird, J.G., et al. 2015. Identification of a VxP Targeting Signal in the Flagellar Na+ /K+ -ATPase.. Traffic, 1239-1253.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
37428632	3ug	Yoon, S., et al. 2023. Early Developmental Deletion of Forebrain Ank2 Causes Seizure-related Phenotypes by Reshaping the Synaptic Proteome. Cell reports, 112784.

Western Blot: Bovine
PMID	Dilution	Publication
26373354	not listed	Laird, J.G., et al. 2015. Identification of a VxP Targeting Signal in the Flagellar Na+ /K+ -ATPase.. Traffic, 1239-1253.

Western Blot: Human
PMID	Dilution	Publication
27001749	1:500	Patzke, C., et al. 2016. Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation.. Journal of Experimental Medicine, 499-515.
24462599	not listed	Trinh-Trang-Tan, M.M., et al. 2014. AlphaII-spectrin participates in the surface expression of cell adhesion molecule L1 and neurite outgrowth.. Exp Cell Research, 365-380.

Western Blot: Mouse
PMID	Dilution	Publication
29956586	1:500	Dash, B., et al. 2018. Nonmuscle myosin II isoforms interact with sodium channel alpha subunits. . Molecular pain, .
25308431	1:500	Carney, K.E., et al. 2014. Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins.. Journal of Proteome Research, 5918-5927.

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Anti-Ankyrin-B Antibody (N105/13)

SKU: 75-144

Product Details

Product Specific References for Applications and Species

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