Products: Primary Antibodies

Anti-Ankyrin-B (N105/13)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-144	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-144	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 203-496 of human Ankyrin-B (also known as ankyrin-2, brain ankyrin and non-erythroid ankyrin, accession number Q01484)
Mouse: 99% identity (292/294 amino acids identical)
Rat: 98% identity (291/294 amino acids identical)
~80% identity with Ankyrin-G

Cross Reactivity:

None Reported

Protein Name(s):

Ankyrin-2 (ANK-2) (Ankyrin-B) (Brain ankyrin) (Non-erythroid ankyrin)

Gene Name(s):

ANK2 ANKB

Antibody Registry ID:

Purified: AB_10673094
TC Supernatant: AB_10675129

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

>200 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Patzke C, Acuna C, Giam LR, Wernig M, Südhof TC.. (2016), 'Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation. .' Journal of Experimental Medicine. 10.1084/jem.20150951.
Laird JG, Pan Y,, Modestou M, Yamaguchi DM, Song H,, Sokolov M, Baker SA.. (2015), 'Identification of a VxP Targeting Signal in the Flagellar Na+ /K+ -ATPase. .' Traffic. 10.1111/tra.12332.
Ho TS, Zollinger DR, Chang KJ, Xu M, Cooper EC, Stankewich MC, Bennett V, Rasband MN.. (2014), 'A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier. .' Nature Neuroscience. 10.1038/nn.3859.
Carney KE, Milanese M, van Nierop P, Li KW, Oliet SH, Smit AB, Bonanno G, Verheijen MH.. (2014), 'Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins. .' Journal of Proteome Research. 10.1021/pr500829z.
Trinh-Trang-Tan MM, Bigot S, Picot J, Lecomte MC, Kordeli E.. (2014), 'AlphaII-spectrin participates in the surface expression of cell adhesion molecule L1 and neurite outgrowth. .' Exp Cell Research. 10.1016/j.yexcr.2014.01.012.
Lysakowski A, Gaboyard-Niay S, Calin-Jageman I, Chatlani S, Price SD, Eatock RA.. (2011), 'Molecular microdomains in a sensory terminal, the vestibular calyx ending. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0521-11.2011.
Huff TB, Shi Y, Sun W, Wu W, Shi R, Cheng JX.. (2011), 'Real-time CARS imaging reveals a calpain-dependent pathway for paranodal myelin retraction during high-frequency stimulation. .' PLoS One. 10.1371/journal.pone.0017176.