Products: Primary Antibodies

Anti-Ankyrin-B (N105/13)

PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-144	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $205.00
Catalog # 73-144	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $180.00

* Prices shown reflect any applicable non-profit/academic discount. NeuroMab antibodies will have higher for-profit/distribution and orders placed online will need to be adjusted. Please inquire with us for more information.

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 20-60 ug/mL monoclonal antibody (mAb). Supernatant is suitable for most applications, including Western Blot, immunocytochemistry, immunohistochemistry, etc., and is a very economical choice compared to competitor offerings. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 203-496 of human Ankyrin-B (also known as ankyrin-2, brain ankyrin and non-erythroid ankyrin, accession number Q01484)
Mouse: 99% identity (292/294 amino acids identical)
Rat: 98% identity (291/294 amino acids identical)
~80% identity with Ankyrin-G

Cross Reactivity:

None Reported

Protein Name(s):

Ankyrin-2 (ANK-2) (Ankyrin-B) (Brain ankyrin) (Non-erythroid ankyrin)

Gene Name(s):

ANK2 ANKB

Antibody Registry ID:

Purified: AB_10673094
TC Supernatant: AB_10675129

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

>200 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.