Anti-GFP Recombinant Chicken Chimeric mAb (N86/38)

Name: Anti-GFP Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-132
Price: 481.0 USD
Availability: InStock

Our chicken Anti-GFP recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Immunofluorescent staining of COS-7 cells transfected with GFP using Aves Labs’ anti-GFP recombinant chicken monoclonal antibody (red, Cat No. 78-132) and showing complete overlap with GFP autofluorescence (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells.

Western blotting of mock transfected and GFP transfected COS-7 lysates with anti-GFP recombinant chicken mAb (Cat No. 78-132) antibody (1 µg/mL) and detected with anti-chicken-HRP. The antibody recognizes exogenous GFP only in GFP transfected COS-7 lysates at ~26 kDa.

Immunofluorescent staining of COS-7 cells transfected with GFP using Aves Labs’ anti-GFP recombinant chicken monoclonal antibody (red, Cat No. 78-132) and showing complete overlap with GFP autofluorescence (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells.

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Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-132

Volume: 100 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GFP

Scientific Background

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N86/38

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

GFP

Molecular Weight

27 kDa

Excitation / Emission

Unconjugated

Immunogen

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Immunogen Species

Jellyfish

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000-1:3000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Does not cross-react with Red fluorescent protein (dsRed)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Green fluorescent protein

UniProt ID

P42212

Shipping

Shipped on ice packs

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Anti-GFP Recombinant Chicken Chimeric mAb (N86/38)

SKU: 78-132

Product Details

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