Anti-Neurofilament light polypeptide (NF-L) Antibody (1B11) (1B11)

Our Anti-Neurofilament light polypeptide (NF-L) mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is IgG. It is validated for use in IF, ICC, IHC, WB.

Image of a rat spinal cord section with a three-day-old midline C4 contusion injury by Immunofluorescence and Immunohistochemistry. The section was stained M-2128-100, Neurofilament light polypeptide (NF-L), Clone 1B11, Mouse mAb, (green), and co-stained with product R-2113-50, Neurofilament light polypeptide, C-terminus, (NF-L-Ct), Rabbit pAb, (red). Product M-2128-100 stains prominent aggregates of material concentrated in the lateral funiculi and dorsal columns, which are degenerating and degenerated axons caused by the C4 lesion. As product R-2113-50 antibody binds the C-terminal "tail" region of NF-L, which is absent or destroyed during degeneration, product M-2128-100 stains positively for degenerating NF-L profiles, but staining with R-2113-50 full-length NF-L is largely negative.
Analysis by western blot of NF-L expression in different tissue lysates with M-2128-100, Neurofilament light polypeptide (NF-L), Clone 1B11, Mouse mAb, (green, 1:20,000) with strong bands at approx 68 - 70kDa. Lane 1: protein standard (red); Lane 2: rat brain; Lane 3: mouse brain; Lane 4: cow cerebellum The lower molecular weight bands detected in the cow cerebellum sample are likely proteolytic forms of NF-L.
Image of a rat spinal cord section with a three-day-old midline C4 contusion injury by Immunofluorescence and Immunohistochemistry. The section was stained M-2128-100, Neurofilament light polypeptide (NF-L), Clone 1B11, Mouse mAb, (green), and co-stained with product R-2113-50, Neurofilament light polypeptide, C-terminus, (NF-L-Ct), Rabbit pAb, (red). Product M-2128-100 stains prominent aggregates of material concentrated in the lateral funiculi and dorsal columns, which are degenerating and degenerated axons caused by the C4 lesion. As product R-2113-50 antibody binds the C-terminal "tail" region of NF-L, which is absent or destroyed during degeneration, product M-2128-100 stains positively for degenerating NF-L profiles, but staining with R-2113-50 full-length NF-L is largely negative.
Image of a rat spinal cord section with a three-day-old midline C4 contusion injury by Immunofluorescence and Immunohistochemistry. The section was stained M-2128-100, Neurofilament light polypeptide (NF-L), Clone 1B11, Mouse mAb, (green), and co-stained with product R-2113-50, Neurofilament light polypeptide, C-terminus, (NF-L-Ct), Rabbit pAb, (red). Product M-2128-100 stains prominent aggregates of material concentrated in the lateral funiculi and dorsal columns, which are degenerating and degenerated axons caused by the C4 lesion. As product R-2113-50 antibody binds the C-terminal "tail" region of NF-L, which is absent or destroyed during degeneration, product M-2128-100 stains positively for degenerating NF-L profiles, but staining with R-2113-50 full-length NF-L is largely negative.
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Bovine, Human, Mouse, Pig, Rat
ICC, IF, IHC, WB
Mouse

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Data Sheet

SKU: M-2128-100

Volume/Size: 100 µg
Price:
$327.00

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