Anti-Autophagy-related protein 3 (ATG3) Antibody

Our Anti-Autophagy-related protein 3 (ATG3) rabbit polyclonal primary antibody detects human, mouse, and rat Autophagy-related protein 3 (ATG3), and is whole serum. It is validated for use in IHC-Frozen, IHC-Paraffin-embedded, WB.

Confocal microscopy on immunofluorescently detected ATG3 in cytospin-isolated human white blood cells using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG3 appear in red . The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. More information on the staining: Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 Alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre-immunisation serum. No staining was observed in HL60 cell line using Rabbit antibody to ATG3: whole serum (R-159-100)
Western blot on mouse brain lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50. Absorbption with the immunising peptide almost removed the three main bands otherwise visible on the unabsorbed strip.
Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50.
Confocal microscopy on immunofluorescently detected ATG3 in cytospin-isolated human white blood cells using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG3 appear in red . The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. More information on the staining: Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 Alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre-immunisation serum. No staining was observed in HL60 cell line using Rabbit antibody to ATG3: whole serum (R-159-100)
Confocal microscopy on immunofluorescently detected ATG3 in cytospin-isolated human white blood cells using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG3 appear in red . The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. More information on the staining: Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 Alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre-immunisation serum. No staining was observed in HL60 cell line using Rabbit antibody to ATG3: whole serum (R-159-100)
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Human, Mouse, Rat
IHC, WB
Rabbit

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Data Sheet

SKU: R-159-100

Volume/Size: 100 µL
Price:
$327.00

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