Monoclonal Antibodies

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Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).Formalin fixed, citric acid treated parafin sections of E18 mouse skeletal muscle. Sections were probed with anti-Actin (AM2021) then anti-Mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-Actin (C-terminal region) Antibody
$292
Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-β-Actin (AM0081) at 1:1000 (lanes 1-6).Immunocytochemical labeling of β-Actin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-β-Actin (clone M008). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-β-Actin Antibody
$379
Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).
PhosphoSolutions Anti-c-Abl (C-terminal region) Antibody
$292
Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser294. The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).
PhosphoSolutions Anti-Progesterone Receptor (Ser294) Antibody
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Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser190. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).
PhosphoSolutions Anti-Progesterone Receptor (Ser190) Antibody
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Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with HSP60 as a loading control.Immunofluorescence of SARS-CoV-2 transfected HEK293 cells showing specific staining of SARS-CoV-2 in red. Nuclei are visualized in blue with DAPI.
PhosphoSolutions Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody
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Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with HSP60 as a loading control.Immunofluorescence of SARS-CoV-2 transfected HEK293 cells showing specific staining of SARS-CoV-2 in green. Nuclei are visualized in blue with DAPI.
PhosphoSolutions Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody
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Immunofluorescence of a section of rat cerebellum selectively labeling the submembraneous cytoskeleton on neurons and cell bodies and dendrites of Purkinje cells with alpha-II-spectrin (Cat. 98-A2SM, 1:2000, green) and labeling the processes of Bergmann glia and astrocytes with anti-GFAP (Cat. 621-GFAP, 1:5000, red). Western blot of rat hippocampal lysate showing specific immunolabeling of the ~240 kDa alpha II spectrin protein.
PhosphoSolutions Anti-Alpha II Spectrin Antibody
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa HSP60 protein.Immunostaining of HeLa cells showing strong mitochondrial labeling of HSP60 (Cat. 750-HSP60, 1:5000, red) and nuclear staining with DAPI (blue).
PhosphoSolutions Anti-Hsp60 Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX1 protein.Immunofluorescence of cultured rat neurons showing nuclear and distal cytoplasmic labeling of FOX1 (Cat. 581-FOX1, 1:500, green).
PhosphoSolutions Anti-FOX1 Antibody
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~85 kDa EWS protein.Immunofluorescence of HeLa cells showing clear localized labeling of the DNA in the nucleus with Anti-EWS (Cat. 540-EWS, 1:1000, green) while Anti-Vimentin (Cat. 2105-VIM, 1:5000, red) labels the cytoplasmic intermediate filament, and Hoechst DNA stain (blue).
PhosphoSolutions Anti-EWS Antibody
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Immunofluorescence of rat cortical neuron-glial cells showing strong cytoplasmic labeling of a small population of developing neurons and their processes with Anti-DCX (Cat. 451-DCX, 1:1000, red) while Anti-MAP2 (Cat. 1100-MAP2, 1:10,000, green) labels dendrites and perikarya or mature neurons, and additional nuclear staining was done with DAPI (blue). Anti-doublecortin is an excellent marker of early developing neuronal cells.Western blot of postnatal day 3 rat brain lysate showing specific immunolabeling of the ~35 kDa and ~45 kDa doublecortin protein.
PhosphoSolutions Anti-Doublecortin Antibody
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Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~62 kDa COBRA1 protein.
PhosphoSolutions Anti-COBRA1 (NELF-B) Antibody
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Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa CD75 protein.
PhosphoSolutions Anti-CD75 (Transferrin receptor) Antibody
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Western blot of rat striatal lysate showing specific immunolabeling of the ~28 kDa Cdk5 protein.
PhosphoSolutions Anti-CDK5 Antibody
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Western blot of UV treated HeLa cell lysate showing specific immunolabeling of the ~98 kDa CD71 protein.
PhosphoSolutions Anti-CD71 Antibody
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Immunofluorescence of a section of rat cerebellum labeled with anti-calbindin (Cat. 302-CALB, 1:2,000, red), colabeled with anti-GFAP (Cat. 620-GFAP , 1:5000,green), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 28 kDa calbindin protein.
PhosphoSolutions Anti-Calbindin Antibody
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (Cat. 2107-VIM, red, 1:2000) and anti-GFAP antibody (Cat. 621-GFAP, green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.
PhosphoSolutions Anti-Vimentin Antibody
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.
PhosphoSolutions Anti-Vimentin Antibody
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Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein.Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 (Cat. 2060-UCHL1, 1:500, red) and anti-Neurofilament H (Cat. 1451-NFH, 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.
PhosphoSolutions Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~ 36 kDa syntaxin 1A protein.
PhosphoSolutions Anti-Syntaxin Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~145 kDa synaptojanin I protein.
PhosphoSolutions Anti-Synaptojanin 1 Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~ 16 kDa synaptobrevin protein. Western blot showing specific immunolabeling of Synaptobrevin (VAMP, cat. 1933-SYB, 1:1000) in mouse brain, rat brain, and lamprey spinal cord. Image kindly provided by Karina Vargas, University of Pittsburgh.
PhosphoSolutions Anti-Synaptobrevin (VAMP) Antibody
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Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa survival motor neuron (SMN) protein.
PhosphoSolutions Anti-Survival Motor Neuron (SMN) Antibody
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