WGA-Biotin, Conjugated
This lectin is useful for the purification of insulin receptors, serum proteins and neuronal tracing. The carbohydrate-binding specificity of WGA has been studied by variety of techniques, such as hapten inhibition of hemagglutination and specific precipitation of glycoconjugate, changes in fluorescence of the lectin (intrinsic) or of chromatogenic ligands (extrinsic), equilibrium dialysis, NMR and x-ray diffraction.
WGA-Biotin, Conjugated
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Ships: 1-2 business days
Histochemistry 1:250-1:1000; WB 1:500-1:2,500; ELISA 1:500-1:2,500.
Dilute biotinylated lectin in PBS containing 0.1 mM calcium chloride ions.
For Histochemistry: the tissues are processed same as for Immunohistochemistry, after blocking step biotinylated lectin is applied, followed by streptavidin conjugated to enzyme and chromogen.
For WB: incubate membrane with blocking protein followed by biotinylated lectin, streptavidin enzyme conjugate and chromogen.
For ELISA: the target proteins are absorbed on ELISA plate at a concentration of 5-10 µg/ml (50-100 µl), followed by blocking with protein solution, Biotinylated lectin, Streptavidin enzyme conjugate, ELISA substrate.
Please refer to Histochemistry, WB and ELISA protocol for detail information.
Please refer to Histochemistry, WB and ELISA protocol for detail information.