Streptavidin/Biotin-ABC-Peroxidase Staining Kit
Our Streptavidin/Biotin-ABC-HRP kit is an ultrasensitive IHC kit to be used for the detection of biotinylated targets (most commonly biotinylated secondary antibodies) and provides sufficient reagent for staining up to 1,000 sections. Every component of the kit, including the Streptavidin-HRP conjugate, are manufactured in-house giving us unrivaled control over product quality and performance.
Procedure: Mixing of HRP streptavidin reagent:
- For IHC or ICC, take 5 mL of PBS without azide; add 0.1 mL of mixing reagent (B) and 0.1 mL of HRP streptavidin reagent (A), mix well.
- If you see high background, 5 mL of PBS without azide 0.1 mL of Reagent B and 0.05 mL of Reagent A
IHC/ ICC Procedure: After biotinylated secondary antibody step, wash slide 5-7X with PBS or washing buffer (this buffer should not contain any azide*), add premixed HRP streptavidin reagent, incubate for 10-20 minutes at RT or 37ºC. Wash with buffer and proceed with chromogen substrate for HRP.
These are guidelines, the optimum dilutions and incubation times for these reagents and reactions should be determined by the individual lab.
*Caution: Peroxidase reagents are destroyed by sodium azide and should be avoided in all buffers and regents.
- Reagent A: 50X HRP Streptavidin Reagent, 2 mL
- Reagent B: 50X Mixing Reagent, 4 mL
Peroxidase block, protein blocking solution, Washing buffer, antigen retrievers, positive or negative control, primary antibody, primary antibody dilution buffer, chromogen, counterstain and mounting medium.