Fast Red Super ™
Our unique Fast Red Super is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC) with alkaline phosphatase.
Fast Red Super ™
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Ships: 1-2 business days
Mixing of Reagent:
- To 4.9 ml of distilled or deionized water in a test tube, add five drops of buffer reagent B, mix well.
- Add Fast Red tablet (Reagent C), let stand in this solution for 5-10 minutes. Vortex for 3-5 minutes till the entire tablet is dissolved.
- Add 5 drops of substrate reagent S, mix.
- After alkaline phosphatase reagent, wash slide with buffer and distilled/deionized water.
- Add few drops of ready to use Fast Red reagent; incubate at RT or 37ºC for 5-10 minutes. The optimum time of incubation for Fast red Super should be determined by the individual lab.
- Wash slides 2-5X with buffer, followed by rinsing with distilled/deionized water.
- Add counterstain compatible with red color. We use hematoxylin for 30-60 seconds, followed by washing with tap water, buffer with pH over 7 to convert purple blue color to blue color.
- The slide can be mounted with aqueous mounting medium. See #6 or organic mounting medium see #7).
- Wash the slide with distilled/deionized water. The slide can be mounted with aqueous mounting medium (we use ImmunoHistoMount cat. # AR-6503; see data sheet).
- Or the slide can be mounted with organic mounting medium. (Dehydrate with increasing % of alcohol, 100% alcohol, followed by limonene, toluene, xylene or any other dehydrating reagent.. (We use Organo/limonene mounting medium cat. # AR-6504, please follow data sheet for mounting medium).