Indo-1, AM Ester Fluorescent Calcium Indicator

Name: Indo-1, AM Ester Fluorescent Calcium Indicator
Brand: Antibodies Incorporated
SKU: 81-3027
Price: 269.0 USD
Availability: InStock

Our Indo-1 AM Ester is a fluorescent calcium indicator that can be loaded into cells via incubation. Calcium imaging is widely used to measure neuronal activity.

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Applications

EM, ICC, IHC

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Data Sheet

SKU: 81-3027

Size: 10 x 100 µg

Variant

Price:

$269.00

Quantity:

Ships: 1-5 business days

Product Details

Scientific Background

Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed.

Applications

EM, ICC, IHC

Molecular Weight

1010 Da

Excitation / Emission

Kd: 250 nM, Ex: 349 nm, Em: 482 nm (no Ca2+); Ex: 331 nm, Em: 398 nm (high Ca2+)

Storage

Store at –20°C, with desiccant; protect from light.

Physical State

Solid

Specificity

Membrane-permeant, ratiometric; can be loaded into cells via incubation.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

Stable for 12 months after date of receipt.

Shipping

Shipped at ambient temperature with desiccant; protected from light

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Indo-1, AM Ester Fluorescent Calcium Indicator

SKU: 81-3027

Product Details

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