Anti-TrpV3 Antibody (N15/4)

Name: Anti-TrpV3 Antibody
Brand: NeuroMab
SKU: 75-043
Price: 329.0 USD
Availability: InStock

Our Anti-TrpV3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N15/4. It detects human, mouse, and rat TrpV3, and is purified by Protein A chromatography. It is great for use in ICC, IP, WB.

Transfected HEK293 cell immunofluorescence staining N15/4 TC supe =green, DAPI=blue.

Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with rat TrpV3 (left lane) or rat Kv2.1 (right lane) plasmids and probed with K89/34 TC supe (left panel) or pure N15/4 (right panel).

Transfected HEK293 cell immunofluorescence staining N15/4 TC supe =green, DAPI=blue.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

Go to Full Product Details

Data Sheet

SKU: 75-043

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

TrpV3

Scientific Background

Transient receptor potential cation channel subfamily V member 3 or TrpV3 is encoded by the gene TprV3. TrpV3 is a member of the transient receptor potential cation channel family. TrpV3 is thought to function in a variety of processes, including temperature sensation and vasoregulation. This channel is activated at temperatures between 22 and 40 °C. TrpV3 is found at the membrane in the CNS, PNS and skin where it acts as a transducer of thermal stimuli. Diseases associated with TRPV3 include several types of Keratoderma.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N15/4

Isotype

IgG2a

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

Gene Name

TrpV3

Molecular Weight

70 kDa

Immunogen

Synthetic peptide amino acids 774-791 (cytoplasmic C-terminus) of rat TrpV3 (KTTLYAFDELDEFPETSV; accession number E9PU00)

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10671952

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Transient receptor potential cation channel subfamily V member 3 (TrpV3) (Vanilloid receptor-like 3) (VRL-3)

UniProt ID

E9PU00

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Rat
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
32938721	1:200	Nguyen, N.D., et al. 2020. Transient Receptor Potential Ankyrin-1 and Vanilloid-3 Differentially Regulate Endoplasmic Reticulum Stress and Cytotoxicity in Human Lung Epithelial Cells After Pneumotoxic Wood Smoke Particle Exposure. Molecular Pharmacology, 586-597.
30895246	1:100	Lu, Y., et al. 2018. Strontium fails to induce Ca 2+ release and activation in human oocytes despite the presence of functional TRPV3 channels. Human Reproduction Open, .

Immunocytochemistry: Mouse
PMID	Dilution	Publication
30895246	1:100	Lu, Y., et al. 2018. Strontium fails to induce Ca 2+ release and activation in human oocytes despite the presence of functional TRPV3 channels. Human Reproduction Open, .

Immunocytochemistry: Rat
PMID	Dilution	Publication
26416880	1:100	Cohen, M.R., et al. 2015. Nerve Growth Factor Regulates Transient Receptor Potential Vanilloid 2 via Extracellular Signal-Regulated Kinase Signaling To Enhance Neurite Outgrowth in Developing Neurons.. Molecular and Cellular Biology, 4238-4252.

Western Blot: Human
PMID	Dilution	Publication
29658714	1:1000	Deering-Rice, C.E., et al. 2018. Activation of TRPV3 by Wood Smoke Particles and Roles in Pneumotoxicity. Chemical Research in Toxicology, 291-301.
23848361	1:1000	Grubisha, O., et al. 2014. Pharmacological profiling of the TRPV3 channel in recombinant and native assays.. British Journal of Pharmacology, 2631-2644.

Western Blot: Mouse
PMID	Dilution	Publication
32097818	not listed	Ardestani, G., et al. 2020. Divalent cation influx and calcium homeostasis in germinal vesicle mouse oocytes. Cell Calcium, 102181.
19091963	1:400	Huang, S.M., et al. 2008. Overexpressed transient receptor potential vanilloid 3 ion channels in skin keratinocytes modulate pain sensitivity via prostaglandin E2.. Journal of Neuroscience, 13727-13737.

Western Blot: Rat
PMID	Dilution	Publication
26416880	1:500	Cohen, M.R., et al. 2015. Nerve Growth Factor Regulates Transient Receptor Potential Vanilloid 2 via Extracellular Signal-Regulated Kinase Signaling To Enhance Neurite Outgrowth in Developing Neurons.. Molecular and Cellular Biology, 4238-4252.

Recover password

Create my account

Anti-TrpV3 Antibody (N15/4)

SKU: 75-043

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-TrpV3 Antibody

Related Products

Recently Viewed