Anti-NSD1 Antibody (N312/10)

Name: Anti-NSD1 Antibody
Brand: NeuroMab
SKU: 75-280
Price: 385.0 USD
Availability: InStock

Our Anti-NSD1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N312/10. It detects human and mouse NSD1, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged NSD1, NSD2, NSD3 or untagged Kv2.1 plasmids and probed with N312/10 (left), 12CA5 (middle) and K89/34 (right).

Representative images of validation of NSD1 (Cat. 75-280, 1:750) protein levels by Western blot in JHU 011, Cal27, and FaDu cell lines with NSD1 knockdown after 72 h doxycycline induction. Image from publication, CC-BY-4.0, PMID: 38539515.

Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged NSD1, NSD2, NSD3 or untagged Kv2.1 plasmids and probed with N312/10 (left), 12CA5 (middle) and K89/34 (right).

Click on image to zoom

Species Reactivity

Human, Mouse

Applications

ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 75-280

Volume: 100 µL

Variant

Price:

$385.00

Quantity:

Ships: 1-2 business days

Product Details

Target

NSD1

Scientific Background

Nuclear Receptor Binding SET Domain Protein 1 is encoded by the gene NSD1. NSD1 is a member of the class V-like SAM-binding methyltransferase superfamily. NSD1 is a histone methyltransferase that is capable of both negatively or positively influencing transcription, depending on the cellular context. NSD1 is expressed in the brain, kidney, spleen, skeletal muscle, thymus, and lung. Diseases associated with NSD1 include Sotos Syndrome 1 and Overgrowth Syndrome.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N312/10

Isotype

IgG2b

Applications

ICC, WB

Host Species

Mouse

Gene Name

NSD1 ARA267 KMT3B

Molecular Weight

300 kDa

Immunogen

Fusion protein amino acids 2129-2696 (C-terminus) of human NSD1 (accession number Q96L73) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_11001827

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Does not cross-react with NSD2 or NSD3

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific (EC 2.1.1.43) (Androgen receptor coactivator 267 kDa protein) (Androgen receptor-associated protein of 267 kDa) (H3-K36-HMTase) (H4-K20-HMTase) (Lysine N-methyltransferase 3B) (Nuclear receptor-binding SET domain-containing protein 1) (NR-binding SET domain-containing protein)

UniProt ID

Q96L73

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PLA: Human
Western Blot: Human | Mouse
Additional Publications: Unspecified

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36206767	not listed	Li, Y., et al. 2022. Histone Methylation Antagonism Drives Tumor Immune Evasion in Squamous Cell Carcinomas. Molecular Cell, 3901-3918.e7.

PLA: Human
PMID	Dilution	Publication
35073946	1:1000	Jevtic, Z., et al. 2022. SMARCA5 interacts with NUP98-NSD1 oncofusion protein and sustains hematopoietic cells transformation. Journal of experimental & clinical cancer research : CR, 34.

Western Blot: Human
PMID	Dilution	Publication
39251788	1:1000	Parolia, A, et al. 2024. NSD2 is a requisite subunit of the AR/FOXA1 neo-enhanceosome in promoting prostate tumorigenesis. Nature Genetics, 2132-2143.
38539515	1:750	Topchu, I, et al. 2024. PIP4K2B Protein Regulation by NSD1 in HPV-Negative Head and Neck Squamous Cell Carcinoma. Cancers, .
35537449	not listed	Drosos, Y., et al. 2022. NSD1 mediates antagonism between SWI/SNF and polycomb complexes and is required for transcriptional activation upon EZH2 inhibition. Molecular cell, .
28067913	not listed	Papillon-Cavanagh, S., et al. 2017. Impaired H3K36 methylation defines a subset of head and neck squamous cell carcinomas.. Nature Genetics, 180-185.

Western Blot: Mouse
PMID	Dilution	Publication
36206767	not listed	Li, Y., et al. 2022. Histone Methylation Antagonism Drives Tumor Immune Evasion in Squamous Cell Carcinomas. Molecular Cell, 3901-3918.e7.
34261657	not listed	Yu, J.R., et al. 2021. The H3K36me2 writer-reader dependency in H3K27M-DIPG. Science Advances, eabg7444.
33626351	not listed	Farhangdoost, N., et al. 2021. Chromatin dysregulation associated with NSD1 mutation in head and neck squamous cell carcinoma. Cell Reports, 108769.
33207202	1:1000	Harutyunyan, A.S., et al. 2020. H3K27M in Gliomas Causes a One-Step Decrease in H3K27 Methylation and Reduced Spreading within the Constraints of H3K36 Methylation. Cell Reports, .

Additional Publications: Unspecified
PMID	Publication
25421653	Satterlee, J.S., et al. 2015. Community resources and technologies developed through the NIH Roadmap Epigenomics Program.. Methods of Molecular Biology, 27-49.

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Anti-NSD1 Antibody (N312/10)

SKU: 75-280

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-NSD1 Antibody

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