Anti-JMJD2A Antibody (N154/32)

Name: Anti-JMJD2A Antibody
Brand: NeuroMab
SKU: 75-189
Price: 329.0 USD
Availability: InStock

Our Anti-JMJD2A mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N154/32. It detects human JMJD2A, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged JMJD2A and untagged Kv2.1 plasmids and probed with N154/32 (left) and K89/41 (right).

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Species Reactivity

Human

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-189

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

JMJD2A

Scientific Background

Lysine Demethylase 4A is encoded by the gene KDM4A. KDM4A is a member of the JHDM3 histone demethylase family. KDM4A is a nuclear protein that contains a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. KDM4A functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. KDM4A is expressed is several organs with the highest expression in the lungs. Diseases associated with KDM4A include Attention Deficit-Hyperactivity Disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N154/32

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

KDM4A JHDM3A JMJD2 JMJD2A KIAA0677

Molecular Weight

120 kDa

Immunogen

Fusion protein amino acids 568-674 of human JMJD2A (accession number O75164) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human

Antibody Registry ID

AB_10671303

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.117

Conjugate

Unconjugated

Specificity

Does not cross-react with JMJD2C

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Lysine-specific demethylase 4A (EC 1.14.11.-) (JmjC domain-containing histone demethylation protein 3A) (Jumonji domain-containing protein 2A)

UniProt ID

O75164

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunohistochemistry: Human
Immunoprecipitation: C. Elegans | Human
Western Blot: human
Additional Publications: Unspecified

Immunohistochemistry: Human
PMID	Dilution	Publication
27626669	not listed	Wang, L.Y., et al. 2016. KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis. Cell Reports, 3016-3027.

Immunoprecipitation: C. Elegans
PMID	Dilution	Publication
21145482	2ug	Black, J.C., et al. 2010. Conserved antagonism between JMJD2A/KDM4A and HP1γ during cell cycle progression.. Molecular Cell, 736-748.

Immunoprecipitation: Human
PMID	Dilution	Publication
27626669	not listed	Wang, L.Y., et al. 2016. KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis. Cell Reports, 3016-3027.

Western Blot: Human
PMID	Dilution	Publication
30057114	1:100	Mishra, S., et al. 2018. Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications. Cell, 803-817.e16.
28905188	1:1000	Neault, M., et al. 2017. miR-137 Modulates a Tumor Suppressor Network-Inducing Senescence in Pancreatic Cancer Cells. Cell Reports, 1966-1978.
27626669	not listed	Wang, L.Y., et al. 2016. KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis. Cell Reports, 3016-3027.
27624942	1:1000	Carbonneau, M., et al. 2016. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway.. Nature Communications, 12700.
26904954	1:1000	Neault, M., et al. 2016. miR-137 Modulates a Tumor Suppressor Network-Inducing Senescence in Pancreatic Cancer Cells.. Cell Report, 1966-1978.
26755726	not listed	Black, J.C., et al. 2016. Regulation of Transient Site-specific Copy Gain by MicroRNA. Journal of Biological Chemistry, 4862-4871.
21757720	not listed	Van Rechem, C., et al. 2011. The SKP1-Cul1-F-box and leucine-rich repeat protein 4 (SCF-FbxL4) ubiquitin ligase regulates lysine demethylase 4A (KDM4A)/Jumonji domain-containing 2A (JMJD2A) protein.. The Journal of Biological Chemistry, 30462-30470.

Additional Publications: Unspecified
PMID	Publication
25421653	Satterlee, J.S., et al. 2015. Community resources and technologies developed through the NIH Roadmap Epigenomics Program.. Methods Mol Biol., 27-49.

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Anti-JMJD2A Antibody (N154/32)

SKU: 75-189

Product Details

Product Specific References for Applications and Species

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