Anti-Gamma-Protocadherin-B2 Antibody (N148/30)

Name: Anti-Gamma-Protocadherin-B2 Antibody
Brand: NeuroMab
SKU: 75-184
Price: 329.0 USD
Availability: InStock

3 Citations

Our Anti-Gamma-protocadherin-B2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N148/30. It is KO validated, detects mouse Gamma-protocadherin-B2, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma-protocadherin-B2 and -B1 and untagged Kv2.1 plasmids and probed with N148/30 (left) and N86/6 (right) TC supe.

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Species Reactivity

Mouse

Applications

ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-184

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Gamma-protocadherin-B2

Scientific Background

Protocadherin Gamma Subfamily B, 2 is encoded by the gene PCDHGB2. PCDHGB2 is a member of the protocadherin gamma gene cluster. PCDHGB2 is a neural cadherin-like cell adhesion protein that most likely plays a critical role in the establishment and function of specific cell-cell connections in the brain. PCDHGB2 is expressed in the nervous system. Diseases associated with PCDHGB2 include Prune Belly Syndrome.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N148/30

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

Pcdhgb2

Molecular Weight

100 kDa

Immunogen

Fusion protein amino acids 719-804 (variable cytoplasmic domain) of mouse Gamma-protocadherin-B2 (accession number Q91XX7) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse

Antibody Registry ID

AB_10675142

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.112

Conjugate

Unconjugated

Specificity

No cross-reactivity against other Gamma-protocadherin-A, -B or -C proteins

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Protocadherin gamma B2 (Protocadherin gamma subfamily B, 2)

UniProt ID

Q91XX7

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
22092001	not listed	Lobas, M.A., et al. 2012. Molecular heterogeneity in the choroid plexus epithelium: the 22-member γ-protocadherin family is differentially expressed, apically localized, and implicated in CSF regulation.. Journal of Neurochemistry, 913-927.
22275881	not listed	Prasad, T., et al. 2011. Direct and Indirect Regulation of Spinal Cord Ia Afferent Terminal Formation by the γ-Protocadherins.. Frontiers in Molecular Neuroscience, 54.

Western Blot: Mouse
PMID	Dilution	Publication
31877124	1:500-1:1000	Garrett, A.M., et al. 2019. CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4. . PLoS genetics, e1008554.

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Anti-Gamma-Protocadherin-B2 Antibody (N148/30)

SKU: 75-184

Product Details

Product Specific References for Applications and Species

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