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Western blot analysis of human cell lysates: A549 (lane 1), A431 (lane 2), LNCaP (lane 3), MDA-MB-231 (lane 4) and MeWo (lane 5). The blot was probed with mouse monoclonal anti-CD99 (CM0261) at 1:1000.Immunocytochemical labeling of CD99 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD99 (clone M026). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of CD99 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD99 (clone M030). The antibody was detected using goat anti-mouse DyLight® 594.
Western blot analysis of human jurkat cells (lanes 1 & 2) and mouse brain (lanes 3 & 4). The blots were probed with anti-Cdc42 antibody at 1:125 (lanes 1 & 3) or 1:500 (lanes 2 & 4).Immunocytochemical labeling in rat PC12 cells grown for 4 days on poly-D-lysine-coated plates in the presence (200 ng/ml) or absence (Control) of Nerve Growth Factor (NGF). Anti-Cdc42 (CM1521) was used at 1:50 dilution followed by labeling with donkey anti-mouse:Cy2.
Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (CM4301) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.
Western blot analysis of human SYF fibroblasts before (lanes 1 & 3) and after (lanes 2 & 4) treatment with alkaline phosphatase. The blots were probed with anti-Cdk1 (N-terminal region) antibody (lanes 1 & 2) or anti-Cdk1 (Tyr-15) phospho-specific antibody (lanes 3 & 4).
Western blot of rat striatal lysate showing specific immunolabeling of the ~28 kDa Cdk5 protein.
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Western blot analysis of human HeLa cells. The blot was probed with anti-Cdk5 (CM2361) antibody at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000.(lane 3).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged CDY1, HA- and Flag- tagged CDYL1 and CDYL2 and untagged Kv2.1 plasmids and probed with N143/36 (left panel), anti-Flag (middle panel) and K89/34 (right panel) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged CDY1, HA- and Flag- tagged CDYL1 and CDYL2 and untagged Kv2.1 plasmids and probed with N143/38 (left panel), anti-Flag (middle panel) and K89/34 (right panel) TC supe.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and low-speed pellet (RBLSP) immunoblot.

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