Anti-Calretinin (CR) Antibody

Our Anti-Calretinin (CR) chicken polyclonal primary antibody detects bovine, human, mouse, and rat Calretinin (CR), and is IgY preparation. It is validated for use in ICC, WB.

A: Adult rat brain hippocampus section stained for calretinin (red) and MeCP2 (green) by Immunohistochemistry. The chicken anti-Calretinin antibody labels a subset of hippocampal interneurons, which also express MeCP2 (R-1810-100). Co-staining is demonstrated in the nucleus by a yellow color. B: Adult rat cortex section co-stained for calretinin (red) and calbindin (M-1797-100, green). Each antibody specifically labels a subset of interneurons (i.e., calretinin-positive or calbindin-postive) that express each marker exclusively. Insets are high-magnification images of the boxed area in each picture. Blue: Nuclear DNA stained with DAPI. IHC method: 45 um sections; tissue was fixed by transcardial perfusion with 4% paraformaldehyde. C: Western blot of proteins parvalbumin (Lane 1), calretinin (Lane 2), calbindin (Lane 3) and rat brain homogenate (Lane 4) with chicken anti-calretinin antibody (1:1,000). Absence of immunreactivity with parvalbumin (Lane 1) and calbindin (Lane 3) demonstrates specificity of this antibody for calretinin (Lane 2). In rat brain, this antibody recognizes a single band at ~32 kDa, corresponding to calretinin.
Left: Staining of rat hippocampus with chicken anti-calretinin (green, 1:1,000) and mouse anti-parvalbumin (M-1813-100, red, 1:1,000) by Immunohistochemistry. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The calretinin and parvalbumin antibodies label different classes of interneurons. Blue: Nuclear DNA stain. Right: Western blot analysis of calretinin expression (green, 1:1,000) in rat brain (2) and spinal cord (3), mouse brain (4) and spinal cord (5), and cow spinal cord (6). A band at 29 kDa corresponds to calretinin protein. The same blot was simultaneously probed with a mouse anti-internexin antibody (red).
A: Adult rat brain hippocampus section stained for calretinin (red) and MeCP2 (green) by Immunohistochemistry. The chicken anti-Calretinin antibody labels a subset of hippocampal interneurons, which also express MeCP2 (R-1810-100). Co-staining is demonstrated in the nucleus by a yellow color. B: Adult rat cortex section co-stained for calretinin (red) and calbindin (M-1797-100, green). Each antibody specifically labels a subset of interneurons (i.e., calretinin-positive or calbindin-postive) that express each marker exclusively. Insets are high-magnification images of the boxed area in each picture. Blue: Nuclear DNA stained with DAPI. IHC method: 45 um sections; tissue was fixed by transcardial perfusion with 4% paraformaldehyde. C: Western blot of proteins parvalbumin (Lane 1), calretinin (Lane 2), calbindin (Lane 3) and rat brain homogenate (Lane 4) with chicken anti-calretinin antibody (1:1,000). Absence of immunreactivity with parvalbumin (Lane 1) and calbindin (Lane 3) demonstrates specificity of this antibody for calretinin (Lane 2). In rat brain, this antibody recognizes a single band at ~32 kDa, corresponding to calretinin.
A: Adult rat brain hippocampus section stained for calretinin (red) and MeCP2 (green) by Immunohistochemistry. The chicken anti-Calretinin antibody labels a subset of hippocampal interneurons, which also express MeCP2 (R-1810-100). Co-staining is demonstrated in the nucleus by a yellow color. B: Adult rat cortex section co-stained for calretinin (red) and calbindin (M-1797-100, green). Each antibody specifically labels a subset of interneurons (i.e., calretinin-positive or calbindin-postive) that express each marker exclusively. Insets are high-magnification images of the boxed area in each picture. Blue: Nuclear DNA stained with DAPI. IHC method: 45 um sections; tissue was fixed by transcardial perfusion with 4% paraformaldehyde. C: Western blot of proteins parvalbumin (Lane 1), calretinin (Lane 2), calbindin (Lane 3) and rat brain homogenate (Lane 4) with chicken anti-calretinin antibody (1:1,000). Absence of immunreactivity with parvalbumin (Lane 1) and calbindin (Lane 3) demonstrates specificity of this antibody for calretinin (Lane 2). In rat brain, this antibody recognizes a single band at ~32 kDa, corresponding to calretinin.
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Bovine, Human, Mouse, Rat
ICC, WB
Chicken

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Data Sheet

SKU: C-1801-50

Volume/Size: 50 µL
Price:
$327.00

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