Anti-Calretinin-binding protein (CR) Antibody (3G9)

Our Anti-Calretinin-binding protein (CR) mouse monoclonal primary antibody detects bovine, human, mouse, and rat Calretinin-binding protein (CR), and is affinity-purified. It is validated for use in IHC-Frozen, WB.

Left and Middle: Detection of calretinin immunoreactivity (red) in adult mouse brain hippocampal sections (Left) and adult rat cortical sections (Middle) by Immunohistochemistry. The calretinin antibody (1:1,000) stains a small number of interneurons in the stratum radiatum of CA1 region (Left), while Fox/NeuN (R-3770-100, green) is expressed in most neurons in the brain. As a result, co-labelled neurons that stain positive for calretinin appear yellow. In rat cortex (Right), calretinin (red) is expressed in a small population of interneurons concentrated in Layer 4 area, while calbindin (C-1798-50, green) is expressed in cells concentrated in Layer 2/3. Because each antibody specifically labels a different population of cells exclusively, the cells are either stained green or red in the cortex. Blue: DAPI nuclear stain. Insets are high-magnification images of the boxed area in each picture. IHC method: 45 um sections, tissue fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calretinin. The antibody binds strongly and cleanly to a calretinin band at ~32 kDa in cow cerebellum homogenate (Lane 1). Specificity for calretinin and absecnce of cross-reactivity with other related calcium-binding proteins is shown by probing recombinant proteins: secretagogin (Lane 2), parvalbumin (Lane 3), calretinin (Lane 4), calbindin (Lane 5).
Rat cerebellum stained with mouse anti-calretinin (green, 1:500) and chicken anti-calbindin (C-1798-50, red, 1:2,000) by Immunohistochemistry. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The calretinin antibody labels interneurons in the granule cell layer, while the calbindin antibody stains Purkinje neurons and their dendritic processes in the molecular layer of the cerebellum.
Left and Middle: Detection of calretinin immunoreactivity (red) in adult mouse brain hippocampal sections (Left) and adult rat cortical sections (Middle) by Immunohistochemistry. The calretinin antibody (1:1,000) stains a small number of interneurons in the stratum radiatum of CA1 region (Left), while Fox/NeuN (R-3770-100, green) is expressed in most neurons in the brain. As a result, co-labelled neurons that stain positive for calretinin appear yellow. In rat cortex (Right), calretinin (red) is expressed in a small population of interneurons concentrated in Layer 4 area, while calbindin (C-1798-50, green) is expressed in cells concentrated in Layer 2/3. Because each antibody specifically labels a different population of cells exclusively, the cells are either stained green or red in the cortex. Blue: DAPI nuclear stain. Insets are high-magnification images of the boxed area in each picture. IHC method: 45 um sections, tissue fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calretinin. The antibody binds strongly and cleanly to a calretinin band at ~32 kDa in cow cerebellum homogenate (Lane 1). Specificity for calretinin and absecnce of cross-reactivity with other related calcium-binding proteins is shown by probing recombinant proteins: secretagogin (Lane 2), parvalbumin (Lane 3), calretinin (Lane 4), calbindin (Lane 5).
Left and Middle: Detection of calretinin immunoreactivity (red) in adult mouse brain hippocampal sections (Left) and adult rat cortical sections (Middle) by Immunohistochemistry. The calretinin antibody (1:1,000) stains a small number of interneurons in the stratum radiatum of CA1 region (Left), while Fox/NeuN (R-3770-100, green) is expressed in most neurons in the brain. As a result, co-labelled neurons that stain positive for calretinin appear yellow. In rat cortex (Right), calretinin (red) is expressed in a small population of interneurons concentrated in Layer 4 area, while calbindin (C-1798-50, green) is expressed in cells concentrated in Layer 2/3. Because each antibody specifically labels a different population of cells exclusively, the cells are either stained green or red in the cortex. Blue: DAPI nuclear stain. Insets are high-magnification images of the boxed area in each picture. IHC method: 45 um sections, tissue fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calretinin. The antibody binds strongly and cleanly to a calretinin band at ~32 kDa in cow cerebellum homogenate (Lane 1). Specificity for calretinin and absecnce of cross-reactivity with other related calcium-binding proteins is shown by probing recombinant proteins: secretagogin (Lane 2), parvalbumin (Lane 3), calretinin (Lane 4), calbindin (Lane 5).
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Bovine, Human, Mouse, Rat
IHC, WB
Mouse

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SKU: M-1799-100

Volume/Size: 100 µg
Price:
$327.00

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