Anti-Calbindin Antibody

Our Anti-Calbindin chicken polyclonal primary antibody detects bovine, human, mouse, and rat Calbindin, and is IgY preparation. It is validated for use in IHC-Frozen, WB.

Left and Middle: Detection of calbindin-immunoreactivity (red) in adult rat brain cortex (Left) and striatum (Middle) by Immunohistochemistry. The calbindin antibody (1:1,000) labels a subset of sparsely-distributed interneurons (calbindin-positive interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Middle). Since neurons also express Fox3/NeuN (M-377-100, green), calbindin-positive cells appear yellow. Insets are high magnification images of boxed area of each image. Blue: DAPI nuclear stain. IHC method: 45 um sections, fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calbindin expression in rat brain homogenate (Lane 1). The antibody (1:5,000) detects one single band at ~30 kDa. Specificity for calbindin is shown by probing related His-tagged calcium-binding proteins (Lane 2: Parvalbumin; Lane 3: Calretinin; Lane 4: Calbindin; 0.5 ug protein per lane). One additional band at ~60 kDa (Lane 4) most likely represents calbindin protein dimer.
Left: Analysis of calbindin expression (green, 1:2,000) in rat cerebellum by Immunohistochemistry. Sections were co-stained with rabbit anti-MeCP2 (R-1810-100, red, 1:5,000). IHC Method: Transcardial perfusion with 4% PFA, post fixation for 24 hours. Sections were cut to 45 um and stained (free-floating). Calbindin, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells. Right: Western blot analysis of rat cerebellum (2), pig hippocampus (3), cow cerebellum (4), parvalbumin (6), calretinin (7), His-tagged calbindin (8). Chicken anti-calbindin (green, 1:5,000) antibody detects calbindin at ~25 kDa in tissue homogenates (2-4). Recombinant human calbindin protein (8) appears at slightly higher molecular weight due to presence of His-tag. The chicken antibody does not cross-react with closely related protein parvalbumin and calretinin.
Left and Middle: Detection of calbindin-immunoreactivity (red) in adult rat brain cortex (Left) and striatum (Middle) by Immunohistochemistry. The calbindin antibody (1:1,000) labels a subset of sparsely-distributed interneurons (calbindin-positive interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Middle). Since neurons also express Fox3/NeuN (M-377-100, green), calbindin-positive cells appear yellow. Insets are high magnification images of boxed area of each image. Blue: DAPI nuclear stain. IHC method: 45 um sections, fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calbindin expression in rat brain homogenate (Lane 1). The antibody (1:5,000) detects one single band at ~30 kDa. Specificity for calbindin is shown by probing related His-tagged calcium-binding proteins (Lane 2: Parvalbumin; Lane 3: Calretinin; Lane 4: Calbindin; 0.5 ug protein per lane). One additional band at ~60 kDa (Lane 4) most likely represents calbindin protein dimer.
Left and Middle: Detection of calbindin-immunoreactivity (red) in adult rat brain cortex (Left) and striatum (Middle) by Immunohistochemistry. The calbindin antibody (1:1,000) labels a subset of sparsely-distributed interneurons (calbindin-positive interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Middle). Since neurons also express Fox3/NeuN (M-377-100, green), calbindin-positive cells appear yellow. Insets are high magnification images of boxed area of each image. Blue: DAPI nuclear stain. IHC method: 45 um sections, fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calbindin expression in rat brain homogenate (Lane 1). The antibody (1:5,000) detects one single band at ~30 kDa. Specificity for calbindin is shown by probing related His-tagged calcium-binding proteins (Lane 2: Parvalbumin; Lane 3: Calretinin; Lane 4: Calbindin; 0.5 ug protein per lane). One additional band at ~60 kDa (Lane 4) most likely represents calbindin protein dimer.
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Bovine, Human, Mouse, Rat
IHC, WB
Chicken

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SKU: C-1798-50

Volume/Size: 50 µL
Price:
$327.00

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