Anti-Calbindin-binding protein Antibody (4H7)

Our Anti-Calbindin-binding protein mouse monoclonal primary antibody detects bovine, chicken, horse, human, mouse, pig, and rat Calbindin-binding protein, and is affinity-purified. It is validated for use in IHC-Frozen, WB.

Left and middle: Detection of calbindin immunoreactivity in rat brain cerebellum (Left) and cortex (Middle) sections by Immunohistochemistry. Calbindin (red) is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum (left), and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex (middle). Methyl-CpG binding protein 2 (Rabbit anti-MeCP2, R-1810-100, green) is universally expressed in the nuclei of almost all neurons. As a result, double-immunofluorescence shows that calbindin-expressing cell somas appear red, but the nucleus appears to be yellow. Blue: DAPI nuclear stain. Insets represent high magnification images of the boxed areas. IHC method: 45 um sections, fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calbindin expression in cow cerebellum homogenate. The antibody shows strong immunoractivity with one calbindin band at ~30 kDa.
Left: Rat brain cerebellum stained with mouse anti-calbindin (green, 1:1,000) and rabbit anti-calretinin (R-1800-50, red, 1:5,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. Calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum. In contrast, the calretinin antibody stains granule cells, in the granualar layer, and their processes in the molecular layer. Right: Western blot analysis of calbindin expression (antibody dilution: 1:2,000) in rat cerebellum (2), pig hippocampus (3), and cow cerebellum (4). The band at ~25 kDa corresponds to calbindin protein, heavily expressed in the cerebellum, but less in hippocampus.
Left and middle: Detection of calbindin immunoreactivity in rat brain cerebellum (Left) and cortex (Middle) sections by Immunohistochemistry. Calbindin (red) is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum (left), and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex (middle). Methyl-CpG binding protein 2 (Rabbit anti-MeCP2, R-1810-100, green) is universally expressed in the nuclei of almost all neurons. As a result, double-immunofluorescence shows that calbindin-expressing cell somas appear red, but the nucleus appears to be yellow. Blue: DAPI nuclear stain. Insets represent high magnification images of the boxed areas. IHC method: 45 um sections, fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calbindin expression in cow cerebellum homogenate. The antibody shows strong immunoractivity with one calbindin band at ~30 kDa.
Left and middle: Detection of calbindin immunoreactivity in rat brain cerebellum (Left) and cortex (Middle) sections by Immunohistochemistry. Calbindin (red) is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum (left), and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex (middle). Methyl-CpG binding protein 2 (Rabbit anti-MeCP2, R-1810-100, green) is universally expressed in the nuclei of almost all neurons. As a result, double-immunofluorescence shows that calbindin-expressing cell somas appear red, but the nucleus appears to be yellow. Blue: DAPI nuclear stain. Insets represent high magnification images of the boxed areas. IHC method: 45 um sections, fixed by transcardial perfusion with 4% paraformaldehyde. Right: Western blot analysis of calbindin expression in cow cerebellum homogenate. The antibody shows strong immunoractivity with one calbindin band at ~30 kDa.
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Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
IHC, WB
Mouse

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Data Sheet

SKU: M-1797-100

Volume/Size: 100 µg
Price:
$327.00

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