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Immunoblot on adult rat brain membrane (RBM), and adult mouse brain membranes (MBM) from wild-type (WT) and Caspr2 knockout mice. Caspr KO sample courtesy of Drs. Ido Horresh and Ori Peles, The Weizmann Institute of Science.Adult rat hippocampus immunohistochemistry.
Western blot of lysed HFF cells that were serum starved for 24 hours and then refed for 30 minutes showing specific immunolabeling of the ~130-260 kDa CaSR protein.
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Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with untagged Cav1.2, Cav1.3 or Kv2.1 plasmids and probed with N263/31 (left) and L57/46 (right) TC supe.TIRF images (top row), super-resolution GSD localization maps (middle row) and binarized images (bottom row) of immunostained CaV1.2 (Cat. 75-257, 1:200) channels and β1ARs in representative fixed adult ventricular myocytes isolated from WT mice under control (A) or ISO-stimulated conditions (B). Merged two-channel image showing relative distributions of CaV1.2 and β1ARs is shown in the third column with the colocalized binarized image. Image from publication CC-BY-4.0. PMID:37438479
Adult rat brain membrane immunoblot.Adult rat hippocampus immunohistochemistry.
Immunostaining of mouse brain cortex identifying the calcium channels along axons stained with Anti-Cav1.3 Ca2+ Channel Antibody (Cat. 75-080, 1:500, green) and nuclei are labeled with DAPI (blue). Image kindly provided by Kaveh Moradi, Virginia Commonwealth University.Adult rat hippocampus immunohistochemistry.

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