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Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).Immunolabelling showing α1-/α5-containing GABAA receptors demonstrated synaptic localizations in a primary hippocampal neuronal culture. Distributions of α1 and α5 (cat. 75-401, 1:1000) subunits (A,B) were co-stained with the inhibitory presynaptic marker protein vGat (C). (D) The α1 subunit was mostly synaptically localized, opposing vGat, whereas α5 was distributed at both the soma and synapse and often showed co-localization with α1-vGat puncta at the synapse (E,F). CC-BY-4.0. PMID:38201218
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with DsRed-tagged GABAARAlpha4, Flag-tagged GABAARAlpha6 or untagged Kv2.1 plasmid probed with N229A/32 (left), Mouse anti-Flag (middle) or N398A/34 (right) TC supe.
Adult rat brain membrane immunoblotAdult rat whole brain immunohistochemistry
Adult rat brain immunohistochemistry.Immunoblot against crude membranes and low- speed pellets from adult rat heart (RHM, RHLSP) and brain (RBM, RBLSP) and extracts of COS cells transiently transfected with HA-tagged GABAAREpsilon or untagged Kv2.1 plasmid probed with N413/67 (left) or N52A/42 (right) TC supe.

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