Anti-Brain-derived neurotrophic factor (BDNF) Antibody (3C11)

Name: Anti-Brain-derived neurotrophic factor (BDNF) Antibody
Brand: Biosensis
SKU: M-1736-100
Price: 417.0 USD
Availability: InStock

Our Anti-Brain-derived neurotrophic factor (BDNF) mouse monoclonal primary antibody detects human, mouse, other mammals (predicted), and rat Brain-derived neurotrophic factor (BDNF), and is IgG. It is validated for use in ELISA, ICC, WB.

Western blot analysis of rat astrocyte culture (Lanes 1 and 2) and rat hippocampal neuron culture lysate (Lanes 3 and 4) at two different antibody concentrations (1 µg/mL and 0.2 µg/mL).Photo courtesy of Koppel and Timmusk (Tallinn Technical University, Institute of Gene Technology).

Anti-Brain-derived neurotrophic factor (BDNF) Antibody

Western blot analysis of rat astrocyte culture (Lanes 1 and 2) and rat hippocampal neuron culture lysate (Lanes 3 and 4) at two different antibody concentrations (1 µg/mL and 0.2 µg/mL).Photo courtesy of Koppel and Timmusk (Tallinn Technical University, Institute of Gene Technology).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1736-100

Volume/Size: 2 x 50 µg

Variant

Price:

$417.00

Quantity:

Product Details

Target

Brain-derived neurotrophic factor (BDNF)

Scientific Background

BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.

Format

IgG

Clonality

Monoclonal

Clone

3C11

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Mouse

Immunogen

Recombinant human mature BDNF expressed in E.coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute each vial with 50 µL sterile-filtered, ultrapure water to obtain a concentration of 1 mg/mL. Centrifuge to remove any insoluble material. Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified mouse IgG.

Buffer

Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.

Dilution Ranges

WB: 0.2-2 µg/mL
IHC: 2-20 µg/mL
ICC: 2-20 µg/mL
ELISA: 50-100 ng/mL

Conjugate

Unconjugated

Specificity

Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Brain-derived neurotrophic factor; Abrineurin; proBDNF

UniProt ID

P23560

Shipping

25°C (ambient)

Technical Note 4 - Acid Extraction of Brain Tissue

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Anti-Brain-derived neurotrophic factor (BDNF) Antibody (3C11)

SKU: M-1736-100

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