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ImmunoChemistry Technologies Recombinant Mouse IL-13

$200

ImmunoChemistry Technologies Recombinant Mouse IL-17A

$200

ImmunoChemistry Technologies Recombinant Mouse IL-18

$200

ImmunoChemistry Technologies Recombinant Mouse IL-2

$200

ImmunoChemistry Technologies Recombinant Mouse IL-34

$200

ImmunoChemistry Technologies Recombinant Mouse IL-4

$200

ImmunoChemistry Technologies Recombinant Mouse IL-6

$200

ImmunoChemistry Technologies Recombinant Mouse M-CSF

$200

ImmunoChemistry Technologies Recombinant Mouse TNF alpha

$200

ImmunoChemistry Technologies Recombinant Mouse TNFSF11 (RANKL)

$200

ImmunoChemistry Technologies Recombinant Mouse VEGF-A

$200

ImmunoChemistry Technologies Recombinant Rat APRIL

$200

ImmunoChemistry Technologies Recombinant Rat CCL4

$200

ImmunoChemistry Technologies Recombinant Rat CXCL9 (MIG)

$200

ImmunoChemistry Technologies Recombinant Rat IL-17A

$200

ImmunoChemistry Technologies Recombinant Rat TNFSF11 (RANKL)

$200

ImmunoChemistry Technologies Recombinant Rat VEGF-A

$200

Figure 1. Excitation and emission spectra. Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay inhibitor probe was reconstituted in DMSO, diluted in an aqueous buffer, and then analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 630 nm. The emission spectrum (orange) was generated using an excitation of 540 nm. The probe optimally excites at 550-580 nm and has a peak emission at 590-600 nm.

Figure 2. Mice were given either a 0Gy or 15Gy whole lung dose of gamma irradiation. SR in vivo probe (Cat. 982/983) was injected and allowed to circulate for 18 hours prior to sacrifice. Paraffin sections were prepared, nuclei stained with DAPI (blue), and lungs imaged with a fluorescence microscope. Caspase activity (red) was induced in irradiated mice compared to control animals. Data courtesy of E. Hernady (University of Rochester Medical Center, Rochester, NY).

ImmunoChemistry Technologies Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay

From $211.70

Figure 1. Control Alzheimer's Disease (AD) macrophages and curcumin-treated AD macrophages were exposed to FITC-labeled Aß and stained with SR-DEVD-FMK (Cat. 931/932). Macrophages that engulf FITC-Aß fluoresce green (right). Caspase-positive cells fluoresce red (left). Essentially all of the untreated AD macrophages engulfed FITC-Aß and became apoptotic. Curcumin-treated AD macrophages engulfed FITC-Aß but did not become apoptotic. Data courtesy of Dr. Milan Fiala, UCLA 072205.

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-3/7 (DEVD) Assay Kit

From $222.50

Figure 1. Suspension Jurtkat cells were treated with DMSO (control, A) or 1 µM staurosporine (B) for 4 hours at 37°C, then stained with SR-LETD-FMK (kit cat. 9149/9150) for 1 hour at 37°C. Cells were washed three times and slides prepared. Treated cells appear bright red, indicating a high level of caspase-8 activity (B). Few non-induced cells are red, indicating minimal caspase-8 activity (A, Non-Induced, left). Data courtesy of Mrs. Tracy Murphy, ICT (220:68, 121815).

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-8 (LETD) Assay Kit

From $223

Figure 1. Jurkat cells, grown in suspension, were incubated with 1 µM staurosporine for 3 hours at 37°C to induce apoptosis, and then labeled with SR-LEHD-FMK (Cat. 960/961) for 60 minutes at 37°C. Slides were prepared and imaged using a fluorescence microscope with a broad band pass filter. On slide B, cells appear very bright red, indicating a high level of active caspase-9 in these apoptotic cells. Non-induced cells did not fluoresce (slide A). Data courtesy of Dr. Brian W. Lee, ICT.

Figure 2. Jurkat cells were exposed to two experimental conditions (A and B). Each condition was treated with either DMSO (negative) or staurosporine (apoptotic), then labeled with SR-LEHD-FMK (Cat. 960/961). Samples were read in a fluorescence plate reader (550 nm excitation and 590 nm emission using a 570 nm cut-off filter). Staurosporine induced caspase-9 activity 3.3X in both conditions: Condition A (11.0 to 36.5) and Condition B (12.7 to 41.8). Data courtesy of Mrs. Tracy Murphy, ICT 8Y18.

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-9 (LEHD) Assay Kit

From $222.50

Figure 1. HL-60 cells were treated with camptothecin (Cat. 6210), followed by SR-VAD-FMK (Cat. 916/917) and green FAM-FLISP serine protease inhibitor FFCK (Cat. 946), and then analyzed on a scanning laser cytometer. Active caspases stain red (X-axis) and active serine proteases stain green (Y-axis). Co-localization of caspase and serine protease activity is evident in B, C, D, and G. Data courtesy of Dr. J. Grabarek, Brander Cancer Center, NY, and Pomeranian School of Medicine, Szczecin, Poland.

Figure 2. Paramecium were starved for 12-24 hours in 5 mM KCl to induce autogamy. SR-VAD-FMK (Cat. 916/917), was added to the live Paramecium for 30 minutes in the dark. Cells were then washed and Hoechst 33342 was added. Cells were incubated in the dark for 10 minutes, washed, and applied to coverslips. Images were captured using a Nikon TE-300 Epi-fluorescent microscope with a SPOT-RT digital camera and merged using SPOT software. Data courtesy of Dr. W. E. Bell, Virginia Military Institute.

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Poly (active) Caspase (VAD) Assay Kit

From $222.50

Figure 1. Jurkat cells were treated with staurosporine (Positive, Induced, right histogram). Negative control cells were spiked with a DMSO vehicle control (Negative, Non-induced, left histogram). Cells were stained with SR-VAD-OPH for 1 hour, washed twice, and read on a flow cytometer. Only 8.0% of control cells displayed caspase activity, whereas staurosporine induced caspase activity in 90.3% of the experimental cells. This is a ratio of 11:1. Data courtesy of Ms. Tracy Hanson, ICT 092713.

Figure 2. Jurkat cells were treated with staurosporine to induce apoptosis, then labeled with SR-VAD-OPH for 60 minutes. Cells were washed twice and wet-mount slides were prepared. A grey DIC image (left) was taken, and red fluorescence (right) was detected using a band pass filter (excitation at 565 nm, emission at 590-600 nm). SR-VAD-OPH reveals complete caspase activation in the form of red fluorescence in all cells. Data courtesy of Ms. Tracy Hanson, ICT (ICT111809; SR_OPH_111_17_18).

ImmunoChemistry Technologies Red Fluorescent SR-VAD-OPH in vitro Poly Caspase Apoptosis Detection Reagent

$572

ImmunoChemistry Technologies Red Fluorescent SR101-Leu-CMK Serine Protease Assay Kit

From $212.20

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