Rhod-590, AM Ester Fluorescent Calcium Indicator

Name: Rhod-590, AM Ester Fluorescent Calcium Indicator
Brand: Antibodies Incorporated
SKU: 81-3013
Price: 469.0 USD
Availability: InStock

Our Rhod-590 AM Ester is a fluorescent calcium indicator that can be loaded into cells via incubation. Calcium imaging is widely used to measure neuronal activity.

Applications

EM, ICC, IHC

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Data Sheet

SKU: 81-3013

Size: 10 x 50 µg

Variant

Price:

$469.00

Quantity:

Ships: 1-5 business days

Product Details

Scientific Background

To study the roles of calcium, a key intracellular messenger in cells, it is essential to quantitatively monitor its concentration. The most widely used method of Ca2+ detection is the use of fluorescent Ca2+ indicators. Rhod-590 AM ester is a membrane-permeant calcium indicator that can be loaded into cells via incubation.. Rhod-590 AM ester itself does not bind Ca2+, but it is readily hydrolyzed to Rhod-590 by endogenous esterases once it enters cells. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed.

Applications

EM, ICC, IHC

Molecular Weight

1204 Da

Excitation / Emission

Kd: 0.61 uM, Ex: 590 nm, Em: 616 nm (no Ca2+); Ex: 595 nm, Em: 626 nm (high Ca2+)

Storage

Store at –20°C, with desiccant; protect from light.

Physical State

Solid

Specificity

Membrane-permeant, non-ratiometric; can be loaded into cells via incubation.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

Stable for 12 months after date of receipt.

Shipping

Shipped at ambient temperature with desiccant; protected from light

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Rhod-590, AM Ester Fluorescent Calcium Indicator

SKU: 81-3013

Product Details

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