Rat GDNF Rapid ELISA Assay

Name: Rat GDNF Rapid ELISA Assay
Brand: Biosensis
SKU: BEK-2230-2P
Price: 787.0 USD
Availability: InStock

The Biosensis GDNF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum. This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF.

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Species Reactivity

Rat

Applications

ELISA

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Data Sheet

SKU: BEK-2230-2P

Volume/Size: 2 Plate Kit

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Price:

$787.00

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Product Details

Product Description

The Biosensis GDNF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples.

This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards.

This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF.

This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.

Scientific Background

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.

Target

Glial cell line-derived neurotrophic factor (GDNF)

Immunogen

Recombinant rat GDNF protein, made in E.coli

Immunogen Species

Rat

Range

7.8 - 500 pg/mL

Sensitivity

Typical limit of detection (LOD) for rrGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).

Specificity

Rat. The antibodies used in this kit detect human and mouse GDNF.

The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF Rapid^™ ELISA kit. All Biosensis GDNF Rapid^™ ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF Rapid^™ ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF Rapid^™ ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.

Species Reactivity

Rat

Applications

ELISA

Method of Analysis

Colorimetric

Sample Type

Cell Lysates, Culture Supernatant, Serum

Synonyms

Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor; ATF; GDNF

UniProt ID

Q07731

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months from purchase.

Storage

Store at 2-8°C.

Shipping

Shipped on ice packs

Rat GDNF Rapid ELISA Assay Protocol
Technical Note 1 - ELISA Validation
Technical Note 2 - ELISA Data Analysis
Calculation of Antigen Amount in Tissues and Lysates

Reagent	1 Plate Kit	2 Plate Kit
GDNF antibody coated 96 well microplate	12 × 8 wells	24 × 8 wells
Assay diluent A (1X)	2 × 25 mL	4 × 25 mL
Recombinant rat GDNF standard	2 × 1000 pg	4 × 1000 pg
Quality Control (QC) Sample	2 Vials (see vial label for amount)	4 Vials (see vial label for amount)
GDNF detection antibody (100X)	1 × 110 µL	2 × 110 µL
Streptavidin-HRP (100X)	1 × 110 µL	2 × 110 µL
Wash buffer (10X)	1 × 33 mL	2 × 33 mL
TMB substrate (1X)	1 × 11 mL	2 × 11 mL
TMB stop solution (1X)	1 × 11 mL	2 × 11 mL
Plate sealer	Supplied

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Rat GDNF Rapid ELISA Assay

SKU: BEK-2230-2P

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