Noggin is a glycoprotein predominantly expressed by the dorsal mesoderm during embryogenesis and is secreted as a covalently linked homodimer. Noggin is essential for cartilage morphogenesis and joint formation. It is an inhibitor of bone morphogenic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Defects in Noggin are a cause of symphalangism proximal syndrome (SYM1), of multiple synostoses syndrome 1 (SYNS1), of the tarsal-carpal coalition syndrome (TCC), of stapes ankylosis with broad thumb and toes, and of brachydactyly type B2 (BDB2).
Purified. The Noggin product consists of 5-25% carbohydrate by weight.
Cell Culture
HEK293
A DNA sequence encoding human Noggin protein (containing the signal peptide sequence and the mature human noggin sequence) was expressed in modified human 293 cells.
Reconstitute with sterile-filtered phosphate-buffered saline to a concentration of 20 µg/mL. Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Lyophilized
>95% purity as determined by SDS-PAGE and visualized by silver stain.
When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
