NisslFluor™ Red will stain will stain the soma and proximal reaches of dendrites, cytoplasm, and nuclei of neurons a fluorescent red color under green light excitation.
Image of mouse brain tissue (Images A, B, C by Immunofluorescence) stained with NisslFluor™ Red (TR-200-NRD).Prominent staining of neuronal soma and dendrites is observed, characteristic of healthy neurons
NisslFluor™ Red will stain will stain the soma and proximal reaches of dendrites, cytoplasm, and nuclei of neurons a fluorescent red color under green light excitation. Nissl substance is not normally found in the axon hillock or the axon itself. The stain will be of high contrast, resolution, and brightness and resistant to fading. Although most visible under green light excitation, this fluorochrome will also be moderately visible under blue light excitation and faintly visible under UV excitation.
Nissl substance, also termed chromophilic substance, exists as granular organelles in neuron cytoplasm. Named after its 19th-century discoverer, neurologist Franz Nissl, it consists of rough endoplasmic reticulum, RNA, and proteins and aids in protein synthesis for cell functions like growth and repair. Predominantly located in the neuron's soma, it's also in proximal dendrites but absent in axons. Its appearance and distribution vary based on the neuron type and activity. For instance, protein-synthesizing neurons like pyramidal cells contain large, basophilic Nissl chunks due to their maintenance needs, whereas sensory ganglion cells have dispersed, powdered forms. Typically, they're uniformly spread in the soma and dendritic regions, but their outward concentration might indicate neuronal damage or diseases, such as through central chromatolysis. Notably, after an axon is severed, its Nissl substance vanishes from the cell body. As a key neuronal activity and damage marker, changes in Nissl substance's size and distribution often signal stimuli response and are evident in conditions like Alzheimer's, where its reduction is linked to cognitive decline.
Healthy neurons: Nuclei, Soma, and Dendrites
Human
Staining Nissl substances are present in neuronal soma and dendrites, not typically found in axons. Note: Like their bright-field counterparts, Nissl stains will stain all cells in the brain, not just the neurons. However, the staining of non-neuronal cells will be limited to their nuclei and a very thin rim of cytoplasm. By contrast, the cytoplasm and proximal dendrites of the larger neurons will be quite prominent and easily noticeable.
All Mammals, Human, Mouse, Rat
ICC, IF, IHC
Product TR-200-NRD will stain will stain the soma and proximal reaches of dendrites, cytoplasm, and nuclei of neurons a fluorescent red color under green light excitation. Nissl substance is not generally found in the axon hillock or the axon itself. Please see our website product listing to download the associated protocol for complete use instructions. The number of slides stained will depend upon the species and size of the sections used and the staining container. Using a typical 50 mL Coplin jar, 160 slides (two mouse brain sections per slide) could easily be stained in one afternoon. The entire kit will stain at least 800 slides.
Fluorescence
Liquid, ready to dilute
Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.
For research use only.
United States
Unopened kit 6 months at 2-8°C protected from light
The stock solution can be stored for up to 6 months after receipt at 2-8°C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures. 1X solutions should be made fresh every day when in use.
Bulk Order NisslFluor™ Red Fluorescent Nissl Stain
Refund Policy
Satisfaction Guaranteed
We stand by our products and are here to ensure that you get the most out of each experiment using our products and reagents.
We produce everything that we sell and are dedicated to creating reliable and high-quality products that further scientific research. All of our products are produced and quality control tested in-house. We will never sell a product unless we can get it to work in our hands. If a product does not perform as expected, please contact us within 12 months of product purchase and we will provide expert troubleshooting tips to support you. If we cannot resolve the issue, we will replace the product at no cost or offer a 100% refund. We are passionate about providing researchers with the highest quality products on the market, reagents you can count on to make your next discovery.
