Nerve growth factor (2.5S), Mouse, Purified Native Protein

Name: Nerve growth factor (2.5S), Mouse, Purified Native Protein
Brand: Biosensis
SKU: PE-019-500
Price: 707.0 USD
Availability: InStock

Nerve growth factor (2.5S), purified mouse native protein for cell culture, ELISA, WB.

NGF-induced differentiation of PC12 cells in culture. PC12 cells were plated on poly-lysine-coated coverslips and treated with 75 ng/mL native mouse NGF for 19 days. Cells start to differentiate after 6 days, forming a dense neurite network. Images were taken with a light microscope at 10X magnification.

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Applications

Cell Culture, ELISA, WB

Host Species

Native

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Data Sheet

SKU: PE-019-500

Volume/Size: 500 µg

Variant

Price:

$707.00

Quantity:

Product Details

Target

Nerve growth factor, beta (Beta-NGF)

Scientific Background

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF (see reference below).

Format

Purified

Applications

Cell Culture, ELISA, WB

Host Species

Native

Storage

Reconstitute with sterile-filtered, ultrapure water to a concentration of 1 mg/mL. Store lyophilized protein at 2-8°C. After reconstitution, store at 2-8°C short term. Store long-term at -20°C to -80°C. Avoid repeated freezing and thawing. See expiration date for shelf-life estimates, actual times may vary depending upon experimental conditions and laboratory handling.

Physical State

Lyophilized

Production Notes

Greater than 90% purity (as determined by SDS electrophoresis)

Buffer

Lyophilized from PBS, pH 7.4 without preservatives.

Application Details

Stimulates neurite outgrowth in rat PC12 cells

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt for lyophilized material. Reconstituted material, 5 days at 4°C, 30 days -20°C, 60 days -70°C for highest activity Avoid repeated freezing and thawing. Use insulated storage containers for best results.

Synonyms

mouse NGF; beta NGF

UniProt ID

P01139

Shipping

25°C (ambient)

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Nerve growth factor (2.5S), Mouse, Purified Native Protein

SKU: PE-019-500

Product Details

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