LipoFluor-P2™ Polar Lipid & Endoplasmic Reticulum Tracing Reagent

Name: LipoFluor-P2™ Polar Lipid & Endoplasmic Reticulum Tracing Reagent
Brand: Biosensis
SKU: TR-603-P2
Price: 297.0 USD
Availability: InStock

LipoFluor-P2™ is a cell-permeant stain for intracellular lipid droplets and the endoplasmic reticulum. This tracing reagent can be used on live and fixed cells and is compatible with other dyes.

Staining in cultured cells with TR-603-P2 tracing reagent (Left: cardiomyocytes; Right: H9c2 cells).

Staining of cultured cells (LNCaP prostate cancer cells)

Staining in cultured cells with TR-603-P2 tracing reagent (Left: cardiomyocytes; Right: H9c2 cells).

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Species Reactivity

All Mammals, Human, Mouse

Applications

ICC, IF

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Data Sheet

SKU: TR-603-P2

Volume/Size: 4 vials x 5.5 µL per vial

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$297.00

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Product Description

LipoFluor-P2™ is a cell-permeant stain for intracellular lipid droplets and the endoplasmic reticulum. This tracing reagent can be used on live and fixed cells and is compatible with other dyes. LipoFluor-P2™ has a large Stokes Shift, shows excellent resistance to photobleaching, and demonstrates low cytotoxicity.

Target

Endoplasmic Reticulum Lipids & Polar Lipid Molecules

Immunogen Species

Human

Specificity

Fluorescent imaging of lipid-rich cellular compartments. Cell penetration and localization of LipoFluor-P2™ has been confirmed in cardiomyocytes, H9c2 cell line and LNCaP prostate cells.

Species Reactivity

All Mammals, Human, Mouse

Applications

ICC, IF

Application Details

Sample types: live cells, fixed cells (4% paraformaldehyde). Serum-free culture medium is recommended for staining, as lipids contained in serum can reduce signal. Please refer to the product manual. Microscopy: Epifluorescence microscopy: YES; Confocal microscopy: YES; Multiphoton imaging: YES; Infrared spectroscopy: NO; Raman spectroscopy: NO: Visualization: Ex/Em: 405 nm / 600 nm. The typical working concentration is 10µM (1:1000 dilution) in an appropriate buffer or cell culture medium. Biosensis recommends that the end user determine optimal dilutions/concentrations.

Excitation / Emission

405 nm / 600 nm.

Method of Analysis

Fluorescence

Sample Type

Cells, Tissues (live or fixed)

Buffer

DMSO

Physical State

Liquid, ready to dilute

Production Notes

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.

Synonyms

LipoFluor-P2™

Usage Statement

For research use only.

Country of Origin

United States

Expiration

If protected from light, the liquid 10 mM stock solution vials are stable for 12 months from date of receipt, at 2-8°C. A vial should be diluted immediately prior to use and should be used within 24 hours for best results.

Storage

The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.

Shipping

25°C (ambient)

LipoFluor-P2™ Protocol

Four 0.5 mL microfuge vials containing 5.5 uL/vial of 10 mM stock solution of Lipofluor™ TR-603-P2 in DMSO.

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LipoFluor-P2™ Polar Lipid & Endoplasmic Reticulum Tracing Reagent

SKU: TR-603-P2

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